Study Of The H-103 Resin Extracts Of Millettla. Nitida Var.hirsutissima Z. Wei On Antitumor Effects And Its Mechanism In Vitro

Objective: Investigating antitumor effects of the H-103REM (H-103 resin extracts of Millettla. nitida var.hirsutissima Z.wei ) in vitro and its mechanism to explore new remedy for cancer.Methods: 1.The growth curve of the human breast carcinoma line MCF-7 and the human lung adenocarcinoma cell line A-549 was obtained by cell counting; 2. Cells were treated with different curcumin of H-103REM for 24, 48, 72 h and the growth inhibition rates of MCF-7 and A-549 were measured by MTT method; 3. H-103REM antitumor mechanism in vitro: (1) After being treated with H-103REM for 48h,morphological changes of apoptosis were observed with microscope after un-staining and HE staining. (2) After being treated with H-103REM for 48h,DNAladder was examined by DNA agarose gel electrophoresis in each groups.(3) After being treated with H-103REM for 36h,activity of Caspase-3 in MCF-7 and A-549 was examined by colorimetric detection assay.Results: 1.Logarithmic growth phase of MCF-7 cells appeared on the third to the fifth day after being cultured, and logarithmic growth phase of A-549 cells is the second to the sixth day after being cultured; 2.H-103REM displayed strong proliferation inhibitory effort in a concentration-time dependent manner on all cell lines studied with 0.125-2mg/mL curcumin. Meanwile, the results also showed that the inhibitory effect of H-103REM on MCF-7 is better than that on A-549. 3. (1) After being treated with H-103REM for 48h, nuclear shrinkage, chromatin condensation and margination, apoptotic bodies were observed with microscope in MCF-7 and A-549 cells followed by HE staining. (2) DNA gel electrophoresis showed that a apoptotic characteristic ladder pattern was observed in 2mg/mL H-103REM groups.(3)Colorimetric detection assay also demonstrated that after being treated with2 or 1mg/mL H-103REM for 36h, the Caspase-3 in MCF-7 and A-549 was significantly activated compared with the untreated control(P<0.001).Conclusion: 1.H-103REM has strong proliferation inhibitory effect in a concentration-time dependent manner against human breast carcinoma line MCF-7 and human lung adenocarcinoma cell line A-549 with 0.125-2mg/mL curcumin, and these inhibitory effects of H-103REM on MCF-7 is better than that on A-549.2. Obvious apoptotic characteristics and the changes of Caspase-3 activity of MCF-7 and A-549 observed after being treated with H-103REM indicated that inducing-apoptosis might be one of mechanisms of H-103REM inhibiting the proliferation of the all cells studied.