Study On The Gene Expression Profile Of Adamantinomatous Craniopharyngiomas Using CDNA Microarray

Objective:The molecular mechanism of the pathogenesis of adamantinomatous craniopharyngiomas is unclear. The study was designed to establish the gene expression profile of adamantinomatous craniopharyngiomas and investigate the diferential gene expression related to adamantinomatous craniopharyngiomas.Methods:The changes of gene expression profile between adamantinomatous craniopharyngiomas and normal tissue of stalk hypophysial were analyzed by cDNA microarray which represented approximately 16450 Unigene that would be tested in the assay.RT-PCR technology were employed to validate the authenticity of cDNA microarray.Results:453 genes that were differentially expressed in tumors and normal tissues were identified;Of which 195 genes were up—regulated and 258 were down—regulated.ADAMDEC1,CGA,HBG2,SCAPER and TRPM2 genes were selected randomly and were testified by Real-Time quantitative PCR.We found that ADAMDEC1,CGA,HBG2 and TRPM2 genes were differential expression gene.Conclusions : several genes are invovled in the pathogenesis of adamantinomatous craniopharyngiomas,however,their relation to the formation of adamantinomatous craniopharyngiomas needs to be declared.