The Fuyuan Capaule Effect On Blood Viscosities,NO,ET,TXB2,6-keto-PGF1a With Qi-Deficiency And Blood Syasis Syndrome And The Study Of Relative Mechanisms

Objective(1)To investigate the changes of blood viscosities,serum NO,plasma ET,TXB2,6-keto-PGF1a of rats with qi-deficiency and blood stasis syndrome.(2)To investigate the mechanisms of the fuyuan capsule effect on the changes.Methods(1)18-month-old healthy male and female Sprague-Dawley(SD) 72 rats were divided into 2 groups randomly: model group and blank control group,rats were induced by exertion, starvation, cold and high fat food , forming models with Qi Deficiency and Blood Stasis syndrome.(2)rats with model group appear the syndrome of qi deficiency,blood stasis and rise of blood viscosities. rats were divided into 6 groups randomly and equally: model group, positive control group (Qi-shen capsule), Fu-yuan capsule large dose group, Fu-yuan capsule middle dose group , Fu-yuan capsule little dose group , blank control group,every group has 12 rats.(3)Fu-yuan capsule group and positive control group (Qi-shen capsule) were respectively fed with Fuyuan capsule and Qishen capsule, and model contrast group and blank control group were fed with 0.9 N.S at the same time.(4)The time about fatigue-resistant were measured ,blood viscosities were measured by revolve viscosities apparatus, ET,6-keto-PGF1αand NO level are measured by Radioimmunoassay after 4 weeks.Results:(1)the time fatigue-resistant in model group were significantly shorter than those in blank control group(P<0.01).(2)the level of blood viscosities in model group were higher than those in blank control group(P<0.05).(3)the level of NO,6-keto-PGF1αin model group were significantly lower than those in blank control group(P<0.05),but the levels of ET and TXB2 in model group were significantly higher than those in blank group(P<0.05).(4)there were significantly negative correlation between the levels of TXB2 and 6-keto-PGF1αin blood serum or TXB2 and NO in blood serum(P<0.001);however, there were significantly positive correlation between the level of TXB2 and ET(P<0.001). (5) compared with Fuyuan capsule little dose group,Qishen capsule group, the time fatigue-resistant of rats were lengthened in Fuyuan capsule large dose group and Fuyuan capsule middle dose group(P<0.05).(6) compared with Fuyuan capsule large dose group,Fuyuan capsule little dose group,Qishen capsule group ,the levels of NO and 6-keto-PGF1αwere significantly raised in Fuyuan capsule middle dose group(P<0.05),but the levels of ET and TXB2 were descended(P<0.05).(7)compared with other treatment groups, the levels of blood viscosities were comprehensively reduced in Fuyuan middle dose group (P<0.05).Conclusions(1)The blood viscosities about rats who were suffering from Qi-deficiencyand blood stasis syndrome was having keeped concentrated,sticky,congelative,assemblily state.(2)The ratio of NO/ET and TXB2/ 6-keto-PGF1αtook place seriously maladjustment,NO and 6-keto-PGF1αlevels markedly decresed,but TXB2 and ET levels remarkablely increased;these facts can reflect the complex situation of maladjustment from prostaglandins metabolism and the unbalance synthesis and secretion of NO and ET.The above factors could relate to VEC damnification.( 3 ) Fuyuan capsule can effectively ameliorate the serious maladjustment of ratio of NO/ET and TXB2/6-keto-PGF1α,raise the levels of NO and 6-keto-PGF1a in blood,meanwhile reduce the levels of ET and TXB2,the above effect of Fuyuan capsule was better than Qishen capsule.the phenomena of Fuyuan capsule could relate to its abilities to improvement of the function and renew of configuration for VEC.