The Effect Of Pridoxamine On Erectile Function In Diabetic Rats

Objective: To investigate the effect of pridoxamine on erectile function in diabetic rats.Methods: 40 male adult Sprague-Dawley rats were induced for diabetes by an intraperitoneal injection of streptozotocin,takeing the blood glucose>16.7mmol/l after 72 hours as standard.Then the diabetic rats were randomly divided into DM group and DM+PM group.The rats in DM +PM group were treated with pridoxamine at a dosage of 200mg/kg through a gastric perfusion every day.Twelve weeks later the erectile function of all these rats were evaluated by an subcutaneous injection of Apomorphine,and their penises tissues were harvested.The NO,NOS, and cGMP levels in corpus cavernosum were measured.Results: The diabetic rat model was successfully established.The erectile function was much better in normal control group (group C,100%) and DM+PM group(71.4%) than in DM group(46.2%),P<0.05;the erected times were 2.30±0.95,1.43±1.16 and 0.54±0.66,P<0.05.The NO level in DM group was significantly decreased(3.23±0.28μmol/gprot), compared with group C(7.82±0.77μmol/gprot) and DM+PM group(6.47±0.54μmol/ gprot),P<0.05.The NOS level in group C(2.00±0.30 U/mgprot) and DM+ PM group(1.07±0.15 U/mgprot) was both much higher than in DM group(0.69±0.11 U/mgprot),P<0.05.The cGMP content in corpus cavemosum was markedly decreased in DM group(36.15±1.94 pmol/ml), compared with group C(54.19±2.62 pmol/ml) and DM+PM group(50.57±1.20 pmol/ml),P<0.05.Conclusion: The pridoxamine has some effect of L-Arg-NO-cGMP pathway on diabetic rats'penis tissues,and may play some role in protecting the harm to erectile function caused by diabeties.