Morphology, Function Studies Of Interstitial Cells Cajal-like Cells In The Guinea Pig Urinary Bladder

Purpose: This subject study the cajal-like interstitial cells(ICCs) in guinea pig bladder. In this exper experiment We observe ICCs utrastructure and the relations of ICCs with the surrounding organization by transmission electron microscope. We record ICCs specificity electric current by cell culture and patch clamp test. In this subject can provide a proof to proved that the ICCs in guinea pig bladder is the pacemaker. It can offer a new starting point linical and treatment of certain diseases (such as the detrusor instability, DI).Methods: In this experimental we observe ICCs ultrastructure and the relations of ICCs with the surrounding organization by transmission electron microscope. By enzyme digestion method and patch clamp technology We observe current characteristics of ICCs in the whole-cell recording mode . It can provide a proof to verify that the ICCs in guinea pig bladder is the pacemaker.Results: In this experimental the ICCs can be observed to have rich in mitochondria, developed the RER, the sliding surface endoplasmic reticulum, Gorky body. It exist a great deal of the gap junction in ICCs-like cells with other cells of the ICCs asked, ICCs-like cells And the smooth muscle cells and nerve cells from formation of membrane. By enzyme digestion method and patch clamp technology We observe current characteristics of ICCs in the whole-cell recording mode . and the specific current of the ICCs are inhibited by pacing Blocking agent ZD7288 in a dose-dependent.Conclusion: The ICCs in guinea pig bladder have a unique ultrastructure, and there is the special relationship between the ICCs in guinea pig bladder bladder with smooth muscle and nerve in the anatomy and the structure. The special relationship ICCs in guinea pig bladder may be the dynamic basis regulate the function of the bladder . We observe current characteristics of ICCs in the whole-cell recording mode . and the specific current of the ICCs are inhibited by pacing Blocking agent ZD7288 in a dose-dependent. This subject proved that the ICCs in guinea pig bladder may be the pacemaker.