The Relativity Between Expressions Of HuR And COX-2 In Epithelial Ovarian Carcinoma And Prognosis Facters

Objective :The aim of this study was to check the expressions of HuR and COX-2 in epithelial ovarian cancer, and examine the relativity between HuR and COX-2.With various clinicopathologic characteristics we investigated the influence of HuR and COX-2 expression on the prognosis and parameter of clinical and pathological and attempt to seek the pathway to prevent the occurrence and development of epithelial ovarian cancer .Methods 1 S-P immunohistochemical technique was used toexamine the expression of HuR and COX-2 in 68 epithelial ovarian carcinoma,10 borderline tumors of the ovary and 5 normal ovary tissues.2 We applied RT-PCR check and measure the expressions of HuR and COX-2 in normal ovarian epithelial tissue and ovarian epithelial cancer tissues .3 The relation of HuR and COX-2 expression with clinical pathological parameter was estimated by correlation analysis.Kaplan-Meier and Cox proportional regression survival analyses were used to compare normal and abnormal expression of HuR and COX-2.Results1 Expression of nuclear HuR in ovarian carcinoma . The nuclear expression of HuR in epithelial ovarian cancer tissue(76.47%)was remarkably higher than that in borderline epithelial ovarian tumor tissues(30.00%)and normal ovarian tissues(0%),and had no remarkably differences between the borderline epithelial ovarian tumor and normal ovarian tissue. (P>0.05)2 Expression of cytoplasmic HuR in ovarian carcinoma .The positive expression rates of cytoplasmic HuR in maglinant,borderline epithelial ovarian tumors and normal ovarian tissues were 45.60%,10%,0% respectively.The cytoplasmic expression of HuR in epithelial ovarian cancer was a significantly higher than that in borderline tumors and normal ovarian tissues and had no remarkably differences between the borderline tumors and normal ovarian tissues .3 Statistical analyses showed that the positive expression rate of cytoplasmic HuR in epithelial ovarian carcinoma was closely correlated with histological grade, and FIGO stage,but not correlated with histological type, lymphnode metastasis and the age of the patients.The rates of expression of cytoplasmic HuR in G2 and G3 groups were higher than that in G1 group (P<0.05), in stagesâ…¢andâ…£were higher than that in stagesâ… andâ…¡(P<0.05). 4 The positive expression rates of COX-2 in malignant ovarian cancer,borderline ovarian tumor and normal ovarian tissues were 67.64%,60.00%,0.00%, respectively .There was a higher difference between malignant,borderline ovarian cancer and normal ovarian tissues,but no remarkably differences between malignant and borderline ovarian tumors .5 COX-2 immunostaining and clinicpathological parameters : statistical analyses showed that the positive expression rate of COX-2 in epithelial ovarian carcinoma was closely correlated with FIGO stage and lymphnode metastasis ,but not correlated with histological type, histological grade and the age of the patients. The rates of expression of COX-2 in stagesâ…¢andâ…£were higher than that in stagesâ… andâ…¡(P<0.05).The cases having lymphnode metastasis had higher COX-2 expression than those having no lymphnode metastasis (P<0.05).6 Correlation between cytoplasmic HuR immunostaining and COX-2 expression : statistical analyses showed a significant correlation between cytoplasmic HuR and COX-2 expressions in epithelial ovarian carcinoma .7 Survival analysis Kaplan-Merier showed that an increased cytoplasmic expression of HuR was a negative prognostic factors for progression-free survival as well as for overall survival(P<0.05).Multivariate Cox regression analysis showed:cytoplasmic expression of HuR was an independent prognostic parameter for reduced overall survival.Other independent parameter were FIGO stage as well as intraoperative residual tumor .Conclusion1 The expression of HuR and COX-2 were higher in the epithelial ovarian carcinoma and the cytoplasmic expression of HuR was significantly correlated with the expression of COX-2. These results suggest that increased cytoplasmic expression of HuR and COX-2 expression may play important roles in the growth and development of epithelial ovarian carcinoma.2 The increased cytoplasmic expression of HuR was associated with survival and prognosis and may be act as a prognostic factor in ovarian carcinoma .