The Application Of Activating Collateral Drugs Treating Stupid Rat Of Blood Vessel Effect Mechanism Studies

Objective: Vascular stupid (Vascular dementia, is called VD) to belong to the category which the senile dementia gets sick. In recent years, along with the social population's aging, the VD prevalence rate assumed year by year the trend of escalation, because its initiation's social question also more and more brought to the people high attention. Therefore, discusses VD thoroughly the pathogenesis, the research and development prevents and controls VD the medicine to have the very vital significance. This research passes winds a kind of traditional Chinese medicine to the VD big mouse blood serum in the third two baldheaded (MDA) the content, the nitrogen monoxide through the observation (NO) the content and the hyper oxide mutate (SOD) the active influence, discusses from the oxidation resistance angle passes winds a kind of traditional Chinese medicine to treat VD the action mechanism, and provides the theory basis for clinical care VD.MethodsUses ties up the two-sided arterial carotids communist anterior repeatedly, simultaneously the abdominal cavity injection niter Punter's method duplicates the VD big mouse model. Divides into 4 groups stochastically 48 healthy female Wister big mice: The Nimmo horizontal control group (English full title control group), the traditional Chinese medicine treatment group (English full title Traditional Chinese medicine treatment group, called treatment group), the model group (English full title model group), the normal control group (English full title normal control group, called normal group), 12/groups. Four group of big mouse compatibilities feed one week later, the control group, the treatment group, at the same time the model group line two-sided arterial carotids communist anterior legation abdominal cavity injection niter Punier duplicates the VD animal model. The normal group does not exert any processing, the control group, the treatment group after the surgery the second day of start, gives Nepal the money even suspension and passes winds The application of activating collateral drugs compound prescription preparation the suspension (leech, earthworm, dead silkworms, woodlouse) to fill the stomach treatment, the normal group and the model group gives the physiological saline to fill the stomach every day. Each group of big mouse beheads draws blood after two weeks, and uses in separately the nitric acid reductive law determination blood serum the NO content, compares in the color analytic method determination blood serum's MDA content, the xanthenes and in the xanthenes enzyme response law determination blood serum the SOD activeness. After right flank taking the brain organization fixed slices, conventional HE dyes with Nepali dyes the manufacture pathological section, observes the morphological change, which under the light microscope each group of big mouse brain organizes.Results1 Passes winds a kind of traditional Chinese medicine to the VD big mouse pathology morphology influence Normal group: Under the light microscope the pathology morphology demonstrated that the big mouse cerebral cortex nerve nuclear group arrange in neat rows, the cell body size is normal, the nucleolus is obvious.Model group: Under the light microscope the pathology morphology demonstrated that the big mouse cerebral cortex nerve nuclear group shape is extremely anomalous, mesenchymal loose, the karyopyknosis becomes the triangle either the multi-distortions, the nucleus dyeing depth or vanishing, cell dropsy is obvious.Treatment group: Under the light microscope the pathology morphology demonstrated that the big mouse cerebral cortex nerve nuclear group arranges neatly, the cell escapes lacks, only has the minority cell karyopyknosis to become the triangle or the multi-distortions, cell dropsy is not obvious.Control group: Under the light microscope the pathology morphology demonstrated that the big mouse cerebral cortex nerve nuclear group arrangement ataxia, the cell escapes loses few, has the most cell karyopyknoses to become the triangle or the multi-distortions, cell dropsy is obvious. 2 Passes winds a kind of traditional Chinese medicine to the VD big mouse blood serum in MDA, SOD, the NO influence2.1 Passes winds a kind of traditional Chinese medicine to the VD big mouse blood serum in the MDA influenceCompared with the model group: big mouse blood serum MDA of treatment group and control group content remarkable drop (P <0.01, P <0.05), and the treatment group is lower than the control group obviously (P <0.01).Compared with treatment group: big mouse blood serum MDA of treatment group and control group content obviously advances(P<0.01),and the control group is higher than the treatment group obviously(P<0.01).2.2 Passes winds a kind of traditional Chinese medicine to the VD big mouse blood serum in the SOD influenceCompared with the model group: big mouse blood serum SOD of treatment group and control group content obvious markup(P<0.01), and the treatment group are higher than the control group obviously(P<0.01).Compared with treatment group: big mouse blood serum SOD of treatment group and control group content obviously drops(P<0.01), and the control group is lower than the treatment group obviously(P<0.01).2.3 Passes winds a kind of traditional Chinese medicine to the VD big mouse blood serum in the NO influenceCompared with model group: big mouse blood serum NO of treatment group and control group content remarkable drop(P<0.01,P<0.05), and the treatment group is lower than the control group obviously(P<0.01).Compared with treatment group: big mouse blood serum NO of treatment group and control group content remarkable markup (P<0.01), and the control group is higher than the treatment group(P<0.01).Conclusion1 Passes winds a kind of traditional Chinese medicine to be possible to reduce in obviously the VD big mouse blood serum MDA, the NO content, enhances SOD the activeness, explained that passes winds a kind of traditional Chinese medicine to be able to reduce the oxidized damage which and to the nerve function hang fire harm obviously the free radical chain-reaction causes.2 Passes winds a kind of traditional Chinese medicine to be able to improve the VD big mouse cerebral cortex's damage obviously.