| Objective:in this study the model of diabetic rats were induced by cavum abdominis administration of streptozotocin(STZ).The DM rats were cured by the Chinese medical therapy of JiPiBuShenHuoXue method(JPM).blood glucose, Proteinuria, blood-fat, renal function,renal pathomorphology,kidney weight/body weight ratio,the level of plasma and renal tissue AngⅡin diabetic rats,the expression of renal TGF-β1 were observed.The effect and mechanism of JPM on the kidney of diabetic rats were investigated so as to provid experimental data.Method:Forty-six healthy male Sprague-Dawley(SD) rats with initial body weights of 180g to 200g were selected to our experiment.After one week, blood glucose and urine glucose were detected.The result was negative.Then they were randomly divided into four groups:Normal group(n=10), Model group,Benazepril group and JPM group(n=12,in each group ).Diabetes were induced by the intraperitioneal administration of streptozotocin(60mg/kg body weight) except the Normal group.Tail vein blood glucose levels were measured after 72h.Animals with blood glucose levels of more than 16.7mmol/l were selected as diabetic rats.An equal volume of citromatic acid buffer was injected into Normal group rats.After three days,Benazepril group:received 10mg/kg Benazepril orally once a day by gastric tube;JPM group:received 7.5g/kg JPM;Model group:received an equal volume of drinking water;Normal group also received an equal volume of drinking water.Animals received standard rat food and water that was available.At 8 weeks after streptozotocin injection,the animals were housed in individual metabolic cages for 24h to obtain urine for the measurement of albumin by radioimmunoassay.The urine creatine(Ucr) was also determined.All animal were killed at 8 weeks after the onset of diabetes,Blood glucose, TC,TG,BUN,Scr were assayed with the automaticbiochemistry analyzer,and calculated Ccr.Plasma AngⅡwas measured by radioimmunoassay,and the two kidney of rats were rapidly removed and weighed , calculated ratio of kidney to body weight.Blood was collected under pentobarbital anesthesia (30mg/kg body weight) for the mimmersed in methyl carnoy's fixative embedded in paraffin,and sectioned for histology.The sections(4um) were stained with hematoxylin and eosin(HE) and periodic acid schiff(PAS),the histological morphology was observed,and used light microscope observe the renal pathological changes. A part of right kidney was used for the measurement of intrarenal angiotensinⅡ,the other part of it Was embedded in paraffin and sectioned for histology,the expression of TGF-β1 was observed with the immunohistochemical staining.The section of HE, PAS staining and immunohisto -Chemistry staining was analyzed with the pathology image analysis system.Results: (1)The plasma glucose levels of diabetic rats were significantly higher than the Normal group after the injection of streptozotocin(P<0.01).JPM could effectively decrease the high plasma glucose level than Model group(P<0.01).There were no significant difference between Benazepril treatment and Model group in level of blood glucose,JPM treatment was more significant than benazepril Benazepril(P<0.01).(2)Their level of TG, TC were higher in diabetic rats than in Normal rats(P<0.01).There were no significant difference between Benazepril rats and Model rats(P>0.05).JPM could significantly decrease the level of TG,TC than Model group(P<0.01).⑶BUN,Ccr and Scr of blood serm in model group were higher than Normal group significantly(P<0.01).BUN,Ccr and Scr of other treatment groups descended significantly than Model group(P<0.01).There were no significant difference between Benazepril group and JPM group (P<0.05).⑷At 8 weeks, the albumin excretion rate of Model group compared with other groups had the unusual significant difference (P<0.01).JPM group could significantly reduce the albumin excretion rate than Model group(P<0.01).There was no significant difference between Benazepril group and JPM group(P>0.05).⑸Kidney weight/body weight ratio were sigificantly increased in Model group than Normal group(P<0.01),and were reduced by treatment of Benazepril and JPM compared the index in Model group(P<0.01).There was no significant differece between Benazepril group and JPM group.⑹The blood and renal tissue angiotensinⅡcontent were higher in Model rats than in Normal rats(P<0.01). angiotensinⅡcontent of Benazepril rats were lower than Model rats(P<0.01).Though JPM could reduce angiotensinⅡcontent,the difference between JPM and Benazepril was significant(P<0.01).⑺The structure of renal glomerular and tubule was clear in Normal group.In Model group, the light microscope observation result showed that:the thickness of glomerular basement membrane,mesangial expansion and deposition of extracellular matrix,proximal epithelia cell degenerated and swelling in renal tubular.There were no such obvious changes between JPM group and Benazepril group.⑻The expression distribution and semi-quantitative analysis of TGF-β1:In Model group,the relative amount and density of TGF-β1 in glomerulus and renal tubule had significant difference with the compared of the Normal group TGF-β1 had strong expression(P<0.01).The expression of TGF-β1 diminution in two treatmentgroups were lower than Model group(P<0.01),but there were no significant difference between JPM group and Benazepril group.Conclusion:1JPM can reduce diabetic rat's blood glucose,improve blood rheology,decrease BUN and Scr to protect renal function.2 JPM can improve the structure of diabetic rat's renal,and decrease angiotensinⅡlevel and inhibit the expression of TGF-β1.3JPM may be similar to Benazepril in the effect of treating diabetic rats,but JPM was superior to Benazepril in reducing blood glucose and in improving blood rheology.
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