A Preliminary Function Study Of CXorf36 Cancer-related Gene

Objective: We find a new cancer-related gene by bioinformatics analysis, CXorf36 (chromosome X open reading frame 36), which is upregurated in many kinds cancer tissue.We testified this phenomenon with RT-PCR in many kinds of tissues and cell lines,but to our surprising ,the mRNA level of CXorf36 in several human cancer cell lines,including three kinds of renal cell carcinoma cell lines is very low. We tried to find out what kinds of cancer which CXorf36 is upregurated.So we can find out a new method of cancer screening .Materials and method:1 Materials: Specimens from the second Hospital of Hebei Medical University tumor surgery from January 2006 to December 2007 in patients with cancer who had been treated with surgery. Hela cervical cancer, liver, HepG2, colon Lovo, A549 lung adenocarcinoma, prostate cancer cell line PC3 form the medical department of Peking University. 786 - O, A498, Caki three renal cancer cell lines form People's Hospital of Beijing University Institute for urinary tract.2 Method: Admitted patients surgical resection of tumor specimens cancer and adjacent normal tissues as control. Divided into five groups, namely 10 cases of liver cancer, cholangiocarcinoma five cases, 15 cases of gastric cancer, colorectal cancer 10 cases, 10 cases of renal cell carcinoma. Tissue specimens by RT-PCR method to detect CXorf36 in each group expression. At the same time specimens by RT-PCR method to detect CXorf36 in Hela cervical cancer, liver, HepG2, colon Lovo, A549 lung adenocarcinoma, prostate cancer cell line PC3 and 786 - O, A498, Caki three renal carcinoma cell line expression.3 Data Processing : The results were analyzed with T-test and chi-square test by SPSS10.0,and we established the standard of statistic significance asα=0.05.Results:1 Expression of CXorf36 mRNA in cancer tissues and normal tissuesThe average expression level of CXorf 36 mRNA in cancer tissues and normal tissues are 4.27士0.87,0.47士0.23. The average expression level of CXorf 36 mRNA in cancer tissues is significantly higher than that in normal tissues(P<0.05). There is significant difference between cancer tissues and normal tissues. The average expression level of CXorf 36 mRNA in each group of tissues are 4.39士0.81,4.10士0.97,4.28士0.69,4.46士0.64,4.09士1.26.There are no significant difference (P> 0.05). The average expression level of CXorf 36 mRNA in each group of normal tissues are 0.49士0.24,0.49士0.29,0.47士0.23,0.46士0.20,0.45士0.28.There are no significant difference (P> 0.05). 2 Expression of CXorf36 mRNA in liver cancer tissues and normal liver tissuesThe average expression of CXorf36 mRNA in liver cancer tissues and normal liver tissues are 4.39士0.81,0.49士0.24. The average expression level of CXorf 36 mRNA in liver cancer tissues is significantly higher than that in normal liver tissues(P<0.05). There is significant difference between liver cancer tissues and normal liver tissues.3 Expression of CXorf36 mRNA in cholangiocarcinoma tissues and normal bile duct tissuesThe average expression of CXorf36 mRNA in cholangiocarci- noma tissues and normal bile duct tissues are 4.10士0.97,0.49士0.29.The average expression level of CXorf 36 mRNA in chola- ngiocarcinoma tissues is significantly higher than that in normal bile duct tissues (P<0.05) . There is significant difference betwe- en cholangiocarcinoma tissues and normal normal bile tissues.4 Expression of CXorf36 mRNA in colorectal cancer tissues and normal large intestine tissuesThe average expression of CXorf36 mRNA in colorectal cancer tissues and normal large intestine tissues are 4.28士0.69,0.47士0.23.The average expression level of CXorf 36 mRNA in colorectal cancer tissues is significantly higher than that in normal large intestine tissues(P<0.05). There is significant difference between colorectal cancer tissues and normal large intestine tissues.5 Expression of CXorf36 mRNA in gastric cancer tissues and normal stomach tissuesThe average expression of CXorf36 mRNA in gastric cancer tissues and normal stomach tissues are 4.46士0.64,0.46士0.20.The average expression level of CXorf 36 mRNA in gastric cancer tissues is significantly higher than that in normal stomach tissues(P<0.05). There is significant difference between gastric cancer tissues and normal stomach tissues.6 Expression of CXorf36 mRNA in renal cancer tissues and normal renal tissuesThe average expression of CXorf36 mRNA in renal cancer tissues and normal renal tissues are 4.09士1.26,0.45士0.28. The average expression level of CXorf 36 mRNA in renal cancer tissues is significantly higher than that in normal renal tissues(P<0.05). There is significant difference between renal cancer tissues and normal renal tissues.7 Expression of CXorf36 mRNA in eight kinds of cancer cell linesThe mRNA level of CXorf36 in several human cancer cell lines,including PC3,LOVO,Hela,A549,HepG2,786-O,A498,Caki cell lines is very low.8 CXorf36 gene cloning and analysis of amino acid sequenceswe got the CXorf36 full-length gene sequences form http://www.genome.ucsc.edu/.we got the Full-length coding sequences by RT-PCR. We forecast the protein containing 182 amino acid and Molecular weight of 20.7 kDa .The genome structure of CXorf36, which contains six serine phosphorylation sites and two threonine phosphorylation sites,have not tyrosine phosphorylation sites.Amino acid sequence alignment indicated that CXorf36 shares 79% identity with mouse hypothetical protein, NP₇80437,which is a putative hydrolase.Conclusion:1 CXorf36 mRNA overexpress in cancer tissues. The over expression maybe contribute to the occurrence of cancer.2 CXorf36 mRNA overexpress in liver cancer, cholangiocarcinoma, colorectal cancer, gastric cancer, renal cancer tissues. Tumor spectrum in CXorf36 are liver cancer, cholangiocarcinoma, colorectal cancer, gastric cancer, renal cancer. CXorf36 maybe the screening as a tumor surveillance, detection tumor invasion and metastasis, as well as predicting the prognosis, early recurrence understanding of the effective index. At the same time CXorf36 for the treatment of tumor invasion and metastasis provides a new target .3 The mRNA level of CXorf36 in several human cancer cell lines,including PC3,LOVO,Hela,A549,HepG2,786-O,A498,Caki cell lines is very low. The gene may belong to the protein expression of interstitial . After the tumor cells in vitro construction human cancer cell lines loss of the gene expression . Tumor cells and stromal cells in primary culture and immunohistochemistry experiments for the study of the gene in tumor tissues and function of the position will be important.