N-acetylcysteine Ameliorating The Organisms' Immunological Inhibition State Of Patients With Progressive Colorectal Carcinoma After Operation

Objective Through detecting some indexes such as CD3+TCR-ξbi-positive T-cell, IL-6,IL-10 and IFN-γ,of the patients with progressive colorectal carcinoma perioperatively,we had demonstrated that after operation,the organisms' immune state have aggravated further because of operation coup and other correlation factors, support the view of tumor-source immunosuppressive.These indexes changed after the operation because of the therapy for antioxidant N-acetylcysteine(NAC),the organisms' immune suppression state was amended,this is the emphasis of the experiment,so it confirm that after operation,the antioxidant NAC could amend the organisms' immune suppression state of the patients with progressive colorectal carcinoma.Method The experiment choose 20 cases in which the patients suffered from colorectal carcinoma and have get radical surgery yet from TIANJIN Union Medicine Centre between October and December in 2006,in which 13 cases were males,7 females,ages from 31 to 77 years old,average 59.9 years.All of them had not accepted radiotherapy or chemical therapy,even any other medicine that can affect immune state.These 20 patients were divided into A and B group,10 cases each group,we took A as control group,and B as experiment group,the patients in B group had get NAC in vein dripping for 7 days after operations,8g everyday.The index including CD3+TCR-ξbi-positive T-cell,IL-6,IL-10 and IFN-γ,were detected both in A and B before operation,the 1st day,the 3rd day,the 7th day after operation respectively.By DIF through flow cytometry to detect CD3+TCR-ξbi-positive T-cell,and by ELISA to detect IL-6,IL-10 and IFN-γ.We use the statistics software SPSSl1.5 to analysis the data,and compare the difference of the index between pro-operation and after the operation,and between A and B group. If P＜0.05 it has statistical significance. Result In control group IFN-γand CD3+TCR-ξbi-positive T-cell after operation were descended,but IL-10 was step up in most time subtotal distinction possess statistical significance,(P＜0.05).In experiment group CD3+TCR-ξbi-positive T-cell show augmented tendency than before operation,moreover difference in the 3rd and 7th day after operation have statistical significance,(P＜0.05).These changes contrast in control group:compared with IL-6 in most of time the index is lower than control group,distinction shows statistical significance.Although compared with after operation IFN-γdecreases like control group,but only in less time it had statistical significance:as the same the level of IL-10 does not have manifest upright tendency,only in the 3rd after operation show obvious difference, (P＜0.05).Compared experiment group and control group,in experiment group IFN-γand CD3+TCR-ξbi-positive T-cell are higher than in control group,and in most time it possess statistical significance,(P＜0.05);IL-6 and IL-10 after operation are lower than control group in most detecting time and the distinctions possess statistical significance.(P＜0.05.)Conclusion It discovered that the organisms development immune suppression state in patients with progressive colorectal carcinoma after operation;the therapy of NAC could amend the organisms' immune suppression state;the dose of 8g/day was safety,and we expect that it could offer a new route for progressive colorectal carcinoma in clinical therapy.