The Effect Of Wenhua Capsules In The Cell Apoptosis And Metabolism Of Human Liver Cancer Cell Line Bel-7402

Objective:To investigate the effect of apoptosis and metabolism of a Chinese medical mixture,WenHua capsules,on Bel-7402 cells.Methods:The experiment was divided into four parts.The first part,Bel-7402 cells were cultured in vitro.To set up three observation groups,that is negative control group,the group being exposed to 150μg/ml concentration of WenHua capsules,the group being exposed to 750μg/ml concentration of WenHua capsules.Apoptosis of Bel-7402 was detected by flow cytometry.The change of cell morphology after being exposed to WenHua capsules was observed by using inverted phase contrast microscope.The second part,the secretion of lactate dehydrogenase was detected by velocimetry.The third part,the secretion of VEGF was detected by enzyme linked immunosorbent assay(ELISA).The fourth part,the secretion of transforming growth factorβ1(TGF-β1)was detected by ELISA.Results:The percentage of apoptosis cells increased on WenHua capsules-treated cells,and the relationship between the percentage and the concentration of WenHua capsules was positive correlation.After being exposed to WenHua capsules,the cells were changed into smaller,or longer,or more round.Some vacuole-like structures could be observed in intracytoplasm of some cells.In the mediam,some cells floated. The higher concentration of WenHua capsules,the more obvious of this kind of phenomenon.The lactate dehydrogenase secretion increased after being exposed to 750μg/ml concentration of WenHua capsules(P＜0.05),but the difference between the group treated with 150μg/ml concentration of WenHua capsules and the negative control group was not obvious(P＞0.05).The TGF-β1 secretion in the group being exposed to 750μg/ml concentration of WenHua capsules increased(P＜0.05)while the change of the VEGF secretion among the three groups was not obvious(P＞0.05).Conclusion:WenHua capsules can induce the apoptosis of Bel-7402 cell.It has strong cytotoxicity to Bel-7402 cells,it can make cells hydropic degeneration,increase the secretion of lactate dehydrogenase and TGF-β1.The effect on the secretion of VEGF was not obvious.