| Objective:An intestinal ischemic reperfusion(I/R)rat model was adopted by blocking the SMA to observe the change of intestinal morphology and permeability,the condition of oxygen free radical damage and apoptosis of intestinal mucosa,then the influence of Liangge San on above-mentioned correlated index.The aim of this study was to evaluat the protective effect of Liangge San on intestinal I/R injury,and its possible mechanisms,in order to provide pharmacological evidence for its new clinical use.Method:A total of 168 male Wistar rats were randomly divided into 5 groups as follows:normal group(without any management),sham operation group(SMA was only isolated without clamping+saline was given once a day after operation),model group(SMA was isolated and clamped+saline was given once a day after operation),Liangge San therapeutics group(SMA was isolated and clamped+Liangge San was given once a day after operation),Liangge San prevention and cure group(Liangge San was given once a day for five days before operation+SMA was isolated and clamped+Liangge San was given once a day after operation).Besides 8 rats were chosen randomizedly as normal group,the other groups divided into 5 groups again according to rats in each group were killed at 3,12,24,48,72 hours after I/R(each n=8).At different time point,Chiu's score,D- lactic acid content ofjanitrix blood plasma,MDA content and SOD activity of intestinal homogenate,apoptotic index,the expression of the apoptotic gene Bcl-2 and Bax in the small intestine cells were measured in all rats of each group.Carried on statistical analysis processing using the SPSS 11.5 statistics software.Resuilts:1.After I/R,the instenal morphology was significantly injuried,Chiu's score and D-lactic acid content of blood plasma in model group were higher than that in other groups(p＜0.05 or p＜0.01),all of them reached the peak at 24 hour.Chiu's score and D-lactic acid content in Liangge San intervention groups were lower than those in model group.2.Compared with normal group and sham operation group,the intestinal MDA content of model group increased after ischemia(p＜0.05 or p＜0.01),it reached the peak at 24 hour after reperfusion,then it decreased gradually;SOD activity of model group decreased and reached the lowest point at 24 hour after ischemia reperfusion(p＜0.05 or p＜0.01).Compared with model group,Liangge San therapeutics group,prevention and cure group could decrease MDA content and upgrade SOD activity significantly.3.The apoptotic index,the expression of Bcl-2 gene and Bax gene in intestinal mucosal cells of rats of model group were obviously higher than that in normal group and sham operation group at 3 hours after I/R(p＜0.05 or p＜0.01).Then the expression of Bcl-2 degraded,the apoptotic index and the expression of Bax upgraded continuely.Afler 24 hours,the expression of Bcl-2 began to rise,the apoptotic index and the expression of Bax began to lower.Compared with model group,the expression of Bcl-2 gene enhanced,the apoptotic index and the expression of Bax gene degraded in Liangge San intervention groups.4.Compared with Liangge San therapeutics group in all the index,the effect of Liangge San prevention and cure group was more remarkable(p＜0.05 or p＜0.01)Conclusion:1.The intestinal I/R injury can destroy the mucosa structure of small intestine,increase the intestinal permeability,destroy the function of intestinal barrier.2.The intestinal I/R in rat could induce the production of oxygen free radical increase, the degree of lipid peroxidation enhancement,the activity of antioxidase attenuation, the expression of Bcl-2 gene increase,the expression of Bax gene decrease,the apoptosis of iniestinal mucosa cells increase notably.3.Administration of Liangge San could alleviate the small intestinal histopathologic damage,decrease the intestinal permeability,ameliorate the function of intestinal barrie,and provide protective effect on intestinal I/R injury in rats.4.Liangge San could protect the intestinal tract injury from the intestinal I/R in rats was possibly dued to clean the oxygen free radical,reduce the degree of lipid peroxidation,improve the activities of antioxidase,inhibit the apoptosis of iniestinal mucosa cells during I/R by up-regulating the expression of Bcl-2 gene and down-regulating the expression of Bax gene. |
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