The Expression Of Gene GRIK2 In Leukemia Patients' Bone Marrow By FQ-PCR Method

Background:Leukemia is the most popular malignant tumor among children and teenagers.The cause of the disease is still not clear.Therefore,better understanding of the mechanism is important to the prevention and treatment of the leukemia.Children's acute lymphoblastic leukemia(ALL) is most commom.Many reports showed that children's ALL contains several chromosome parts deficiency,so it's resurable to hypothesize districts which may exist the tumor repressor gene(TSG).Our experiment is a separation and function research of the candidate TSGs which locate on 6q16.3-6q21 deletion segments.My experiment mainly investigated that GRIK2 gene as the candidate for election TSG of ALL, the expression circumstance amomg cell lines,patients' and the control groups' bone marrows through the examination.Objective:To observe GRIK2 gene's expression circumstance among leukemia cell lines,patients'and control groups' bone marrow,I will analysis the expression difference of the GRIK2 gene outcome in bone marrow. Methods:Adopt methods of RT-PCR and FQ-PCR to examine 3 leukemia cell lines,100 patients' and 30 control groups' bone marrow about GRIK2 expression.Use SPSS 14.0 software to do x~2 test,P＜0.05 was considered to be significance.Results:1.Using RT-PCR method to examine 100 patients' bone marrow,the positive ratio of GRIK2 expressed is 3%(3 samples).None of the 3 leukemia cell lines was positive.The positive ratio of GRIK2 expressed in 30 control groups' bone marrow is 10%(3 samples).By covariance analysis,their differences had no statistics meaning(P＞0.05).2.Using FQ-PCR method to examine 100 patients' bone marrow,the positive ratio of GRIK2 mRNA expressed is 6%(6 samples).Only Jurkat cell line of 3 leukemia cell lines is positive.The positive ratio of GRIK2 mRNA expressed in 30 control groups' bone marrow is still 10%(3 samples).The highest content of the GRIK2 mRNA is 2.0606,lowest is 1.8164,mean is 1.9680±0.8901.Conclusion:1.I first step think that the expression of GRIK2 gene between leukemia cells and normal blood organization has no difference.2.The GRIK2 mRNA expresses higher in T-ALL of no 6q deficiency than B-ALL;while the expression is negative in both T-ALL and B-ALL that have the 6q deficiency.3.The FQ-PCR method that examines the GRIK2 mRNA has the characteristics such as convenience,shortuct and high sensitivity, compared the PT-PCR which can reflect the content of GRIK2 mRNA in bone marrow more precisely.4.GRIK2 gene whether or not is TSG of ALL needs the further study. We can adopt more advanced experiment techniques,such as the fluorescence in situ hybridization(Fish),gene expression profile,etc.