Study On Multiple-drug-resistant Genes Of Enterobacter Cloacae In Postoperative Infection Of Liver Transplantation |
Posted on:2009-05-26 | Degree:Master | Type:Thesis |
Country:China | Candidate:F H Luo | Full Text:PDF |
GTID:2144360245983184 | Subject:Clinical Laboratory Science |
Abstract/Summary: | PDF Full Text Request |
Objective: To investigate multiple-drug-resistant genes antibiotic-resistance of Enterobacter cloacae strains after liver transplantation infection as well as their relationship and to provide evidence for prevention against and treatment of infect ion after liver transplantation.Methods: 40 strains of Enterobacter cloacae after liver transplantation infection were collected. The minimal inhibitory concentrations (MICs) of them were determined by standard agar dilution. Cephalosporinase (AmpC)β-lactamase and ESBLs were dectected by three-dimensional tests. MBL were detected by comparing the inhibition zones using imipenem containing EDTA. Genotypes and distribution were determined by polymerase chain reaction(PCR) with type-specific primers and sequence analysis.Results: Sensitivity test shows that they are resistant to the most antibiotics. 22 strains of them carried ESBLs gene: SHV-2, CTX-M-3 and CTX-M-9; 14 isolates carried ampD and ampC gene. 2 isolates carried Metallo-beta-lactamase gene IMP. 28 isolates carried class 1 integron.Conclusion: This investigation shows that there are multiple-drug-resistance and high degree of drug resistance on E. cloacae isolates in postoperative infection of liver transplation which genes of IMP in E. cloacae was firstly reported in China.
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Keywords/Search Tags: | Enterobacter cloacae, multiple-drug-resistance gene, Metallo-beta-lactamase, liver transplantation |
PDF Full Text Request |
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