Changes In Peripheral Blood T Lymphocyte Subsets Of Rabbits In Early Stage After Transplantation Of Tissue Engineered Bone Marrow

Changes in peripheral blood T lymphocyte subsets of rabbits in early stage after transplantation of tissue engineered bone marrowObjective To observe the changes in the peripheral blood T lymphocyte subsets,and to estimate the immunogenicity of tissue engineered bone marrow.Methods Conventional methods of cultivating the BMSCs with SIS composite (M1),and for induced ostcoblast BMSCs with SIS composite(M2)implanted to subcutaneous of rabbits(n=7)and SIS alone was implanted as a control.The flow cytometry was used to observe the changes in the T lymphocyte subsets CD4~+/CD8~+ ratio in the rabbit peripheral blood at before operation,1,3 days and 1,2,4 weeks after the operations.Results The peripheral blood T lymphocyte subsets CD4~+/CD8~+ ratio of M1,M2 groups had no significant difference with the empty contrast group(P＞0.05)in all the stage.Conclusion The change of peripheral blood T lymphocyte subsets suggest the rejection is significant low,which indicate the tissue engineered bone marrow has very low immunogenicity.