The Empirical Study For Tissue Engineered Biological Activity Periosteum Used In Spinal Fusion Of Lumbar Intertransverse Process In Rabbits

The Empirical Study for Tissue Engineered Biological Activity Periosteum Used in Spinal Fusion of Lumbar Intertransverse Process in Rabbits[Objective]to appraise the effect of out-of-body construction tissue engineered biological for bone transplantation surrogate using and spinal fusion of lumbar intertransverse process in rabbits.[Method]out-of-body construction tissue engineered biological implant in 24 healthy adult New Zealand rabbits of lumbar intertransverse process.Each rabbit is taken three transverse process gaps LeftL_4,5,6,RightL_4,5,6.implant Three kinds of materials: imbedding RightL_4,5the induced bone mesenchymal tissue stem cells combination with the pig's small intestine submucosa SIS(A);imbedding RightL_5,6the cytoskeleton(B);imbedding the LeftL_5,6autoallergic flank bone.,Operation the rabbits after 12W,observe the manual palpation,radiography,histology test.[results]manual palpation compared,A,C hasn't statistically significant,but each other has statistically significant with B.By radiography observation,A,C groups' lumbar intertransverse has bone trabecula significantly,but B hasn't bone density.A group I-ollagen,osteocalcin immunohistochemistry dyeing is hadro-masc,which has statistically significant with C,but B group hasn't masc.[Conclusion]Using SIS to compound induced bone mesenchymal tissue stem cells out-of-body construction tissue engineered biological is good surrogate.