Effects Of Profound Hemodilution On Regulatory T Cells And Tissue Metabolism Of Rats

Objective To study the effects of profound hemodilution with oxygen-carrying liquid on regulatory T cells and tissue metabolism of rats.Methods Twenty male adult SD rats weighting 350～400g were used in this study.The animals were anesthetized with intraperitoneal pentobarbital.Arteria carotis communis and femoral vein were cannulated for HR and BP monitoring.Blood was withdrawn from artery and simultaneously replaced by intravenous infusion of equal volume of 6% HES and oxygen-carrying liquid until hematocrit was 10%～20%.Blood samples were taken before hemodilution and end of hemodilution for determination of regulatory T cells by flow cytometer and analysis blood gas.After twenty-four hours, the animals were killed and the heart,lung,liver and kidney of animals were gained to put into the container with formalin,the materials were performed pathological section. Results There were seventeen rats were survival and hematocrit reached objective value(17.41±1.28)%.Hematocrit was significantly attenuated(P＜0.01),CD4~+CD25~+ and oxygen partial pressure were significantly increased(P＜0.01),the pathological section disclosed that the tissues were non-anoxyaemia and non-edema.Conclusion The regulatory T cells of rats could be significantly increased through profound homedilution,haemodynamics were tabilization and the tissues were non-anoxyaemia and non-edema,The oxygen partial pressure of rats may significantly higher by using oxygen-carrying liquid.