Effect Of Gastrodin On Expression Of Cx43 And P38 In Temporal Lobe Cortex And Hippocampus Of PTZ-induced Epileptic Immature Rats

Objective On the basis of the establishment of pentylenetetrazole ignited immature rat model induced seizures,to observe the behavior change and the expression of Cx43and P38 in temporal lobe and hippocampus of each group of rats and its intervention study of Gastrodin.Methods Fifty growth period Wistar rats(50-70g)were randomly divided into five groups,each 10,there were control group,pentylenetetrazole group,gastrodin high-dose group,gastrodin low-dose group and valproate sodium group.According to racine classification scheme for observing the behavior changes and record seizures rank and frequency of experimental rats.To adopt conventional method to perfuse cordis,fix and extract the brain,and the immunohistochemistry was used to detect Cx43 and P38 expression in the temporal lobe and hippocampus of the brain after 4 weeks.Results(1)After two weeks of the experiment,attack rate was 100%in pentylenetetrazole group,attack rates in gastrodin low-dose group,high-dose group and valproate sodium group were all lower than PTZ group(P=0.03,0.003, 0.003);After 4 weeks,attack rate in gastrodin low-dose group and high-dose group were compared with PTZ group(P_α＞0.05),the difference was not statistically significant,but valproate sodium group was lower than that of PTZ group(P＜0.05),the difference were statistically significant;After 4 weeks,ignited rate in PTZ group was 100%,ignited rate in high-dose group and valproate sodium group were lower than PTZ group(P＜0.01).(2)Four weeks later,there was significant difference in comparison among seizures level in each group of experimental rats (χ~2=35.83,P＜0.01),attack level was the highest in PTZ group(Mean Rank= 44.60),followed by gastrodin low-dose group(Mean Rank=34.90),gastrodin high-dose group(Mean Rank=21.45)and valproate sodium group(Mean Rank= 19.55).(3)Four weeks later,rats who were ignited by pentylenetetrazole in all groups, Cx43 expression in temporal lobe cortex and the hippocampus of PTZ group was more than other groups(P_α＜0.01),the difference was statistically significant,and Cx43 expression in gastrodin high-dose group was less than gastrodin low-dose group (q=2.811,2.647 P_α＜0.05),but Cx43 expression in high-dose group compared with valproate sodium group(q=1.203,1.116 P_α＞0.05),there was no statistically significant.(4)Four weeks later,rats who were ignited by pentylenetetrazole in all groups,P38 expression in temporal lobe cortex and the hippocampus of PTZ group was more than other groups(P_α＜0.01),the difference was statistically significant,and P38 expression in gastrodin high-dose group was less than gastrodin low-dose group(q=2.705,2.351 P_α＜0.05),but P38 expression in high-dose group was compared with valproate sodium group(q=1.194,1.031 P_α＞0.05),there was no statistically significant.Conclusion(1)The expression of P38 and Cx43 was increased which may be the cause of PTZ induced epilepsy.(2)Gastrodin could reduce the susceptibility of epileptic seizures and inhibited Cx43 expression of temporal lobe and,hippocampus, inhibited the formation of abnormal gap junction and achieved antiepileptic formation.(3)There was mossy fiber sprouting and the formation of synaptic reconstruction in temporal lobe and hippocampus of the growth period rats who were repeatedly ignited by pentylenetetrazole.Gastrodin have the role to the formation of antiepileptic obviously,and through decreasing the expression of P38,which can inhibit the formation of synaptic reconstruction and control seizures indirectly.