Functional Proteins Expressed In Border Zone After Acute Myocardial Infarction And Their Potential Involvement In Myocardial Homing Of Mesenchymal Stem Cells-a Proteomics Analysis

Objective To utilize differential proteomics on the differential expression of functional proteins in border zone - target zone of mesenchymal stem cells homing after acute myocardial infarction,aiming at understanding the molecular mechanism underlying myocardial homing of MSCs regulated by those functional proteins.Methods AMI was induced by the ligation of rat left anterior descending coronary artery(LAD).After which,group A0,A1,A4,A10 were established successively.Myocardial protein was extracted from border zone and separated in two-dimensional gel electrophoresis.Those spots,which exhibited expression change in time course of myocardial homing of MSCs after AMI,were chosen and identified.Based on bio-information analysis,functional protein potentially involved in homing process were subsequently searched. Proteomics results were further validated both in western blotting and immunohistochemistry.Results Evident time course of histopathological evolution were observed after rat AMI.Among protein spots exhibited significant differential expression in border zone,42 spots were found to evolve as myocardial homing of MSCs,of which,32 spots were identified,representing 29 proteins.For validation,three proteins, CRMP2,prohibitin and cofilin-1,was chosen to undertake the subsequent protein location(immunohistochemistry)and expression (western blotting)study,and the results turned out to be basically supportive.In the subsequent bio-information analysis,those differential proteins were divided into 5 functional groups:sarcomere and cytoskeleton,stress response,energy metabolism,inflammation and miscellaneous.Furthermore,CRMP2,PP2A,subunit B,B-alpha isoform, RACK1 was considered to be potentially involved in myocardial homing of MSCs.Conclusions Differential proteomics may provide a new potential tool in the study of myocardial homing of MSCs.In this study, CRMP2,PP2A,subunit B,B-alpha isoform and RACK1 was finally considered as candidate functional proteins involved in myocardial homing of MSCs.