| Objective: To construct lentiviral vector carrying the Angiopoietin-1(Ang-1) gene, and then infect the rat mesenchymal stem cells (rMSCs) to get Ang-1 gene-modified rMSCs. To transplant Ang-1 gene-modified rMSCs to the atherosclerosis model of rat, then observe the influence of Ang-1 gene modified rMSCs on the carotid atherosclerosis.Methods: the three plasmids system of lentiviral vector: PNL- Ang1 -IRES2-EGFP, HELPER and VSVG were co-transfected to 293T cells to produce Ang-1 gene and the reporter gene enhanced green fluorescent protein (EGFP) gene co-expressing lentivirus. Rat carotid atherosclerosis model was established by balloon injury and high fat diet. rMSCs was collected from bone marrow, cultured and passaged in vitro, then purified by adhesive-screening method. Animals were randomly divided into 3 groups: operation alone group (n=8),pNL-rMSCs transplantation group,(n=8) and Ang-1 gene modified rMSCs transplantation group (n=8).2 weeks and 2months later, the influence of transplantation of gene modified rMSCs on carotid atherosclerosis and in vivo cells differentiation were investigated. The intima-media thickness was observed by hemetoxylin and eosin staining.Results: After three plasmids of lentiviral vector co-transfecting to the 293T cells, a great mount of green fluorescence in 293T cells was observed with the fluorescent microscope. After the constructed lentivirus infected rMSCs, the green fluorescence in rMSCs were observed. The genetic engineering rMSCs could differentiated into cell lineage that obtain endothelial mark; there was not significantly difference in intima-media thickness between the pNL-rMSCs transplantation group and the operation alone group(P> 0.05 ); compared to the rMSCs group, transplantation of Ang-1 gene modified rMSCs can significantly decrease the intima-media thickness(P<0.05)Conclusion: The transplantation of Ang-1 gene modified rMSCs can significantly decrease the atherosclerosis.
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