| Objective To investigate the role of nuclear factor-kappa B(NF-κB) on the expression of parathyroid hormone receptor (PTHR1) and serum calcium level with severe acute pancreatitis (SAP) rats.Methods 72 male Sprague-Dawley rats were randomly divided into sham operation group (SO), SAP group, pyrrolidine dithiocarbamat (PDTC) pretreated SAP group (SAP+PDTC), 24 rats each group. SAP model was developed by injecting 5% sodium taurodeoxycholate into biliary-pancreatic duct. Serum calcium, the expression of NF-κB and PTHR1 by western blot and the expression of PTHR1 by RT-PCR were determined at 0.5, 1, 3, 6 hour after operations.Results NF-κB is activated soon after SAP and reaches the peak at 3 hour, then the expression of PTHR1 in bone and kidney down regulates. Serum levels of calcium is significantly reduced after 6 hour. As compared with SAP group, the level of NF-κB is decreased and the levels of PTHR1 and serum calcium are increased markedly in SAP+PDTC group.Conclusion NF-κB may down regulate the expression of PTHR1 in bone and kidney through complicated interreactions among many kinds of cytokines, leading to the hypocalcaemia of SAP. |
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