Experimental Study Of The Effect Of Different Degrees Of Obstructive Jaundice On The Rats Post-hepatectomy Liver Regeneration

Objective To approach the effect of different degrees of obstructive jaundice on the post-hepatectomy of rats liver regeneration., and provide a theoretical basis for clinical application.Methods Spragure-Dawley rats model of obstructive jaundice was set up and used in measurement at different time points of the level of jaundice. Different groups were divided in accordance with the level of jaundice. 1. SO-Hx groups: normal rat partial hepatectomy groups, 2. OJ1-Hx groups: one days on obstructive jaundice of rat partial hepatectomy groups. 3. OJ3-Hx groups: three days on obstructive jaundice of rat partial hepatectomy guoups.4. OJ7-Hx groups: seven days on obstructive jaundice of rat partial hepatectomy groups; After the operation, the rat mortality, the ratio of residual liver weight / body weight, serum liver function, immunohistological analysis of PCNA index and semiquantitative RT-PCR to determine the HGF mRNA expression in liver tissues were observed.Results After biliary obstruction was induced by common bile duct ligation,serum total bilirubin of SD rats was elevated to the peak (about 85 times) of jaundice at 168 hours of biliary obstruction, and then slow decline. The mortality rate of partial hepatectomy rats were increased after biliary obstruction . The mortality rates of one days on obstructive jaundice, three days on obstructive jaundice, seven days on obstructive jaundice were 6.67%, 26.67%, 46.67% and significantly higher than the control group(0%).The ratio of the right lobe of the liver weight / body weight of the OJ3-Hx group and the OJ7-Hx group were significantly lower than the control group, and their difference were statistical significance(P<0.05).While the OJ1-Hx group was close to the control group, and their difference were not statistical significance(P>0.05).The post-hepatectomy liver function of OJ3-Hx group OJ7-Hx group slowly recovered, and the difference of OJ3-Hx group and OJ7-Hx group compared with the control group from 0 hour to 168 hours after hepatectomy were statistical significance(P<0.05). However, the liver function of OJ1-Hx group relatively smoothly recovered, and the difference of OJ1-Hx group compared with the control group at 168 hours of post-hepatectomy was not statistical significance(P>0.05). Both peak and peak deterioration of PCNA labeling index and HGF mRNA expression in liver tissues of OJ3-Hx group and OJ7-Hx group were weaker, and their difference of OJ3-Hx group and OJ7-Hx group compared with the control group were statistical significant (P <0.05).However , PCNA labeling index and HGF mRNA expression in liver tissues of OJ1-Hx group was close to the control group ,and their difference at 168 hours of post-hepatectomy was not statistical significance(P>0.05). Conclusions 1.The obstructive jaundice rats model was induced by common bile duct ligation bile could simulate some pathophysiological features of obstructive jaundice of hilar cholangicarcinoma. The operation was reduplicative and stabile. 2.Hyperbilirubinemia caused by biliary obstruction obviously inhibited the post-hepatectomy liver regeneration. Inhibitory degree was related with obstructive time and the level of jaundice. And the inhibitory action was inhanced with prolonged obstructive time and elevated level of jaundice.3.Hepatectomy was considered relatively safe at a relatively low level of preoperative bilirubin and short time obstruction.