Expression Of HIF-1 A In The Endometrial Adenocarcinoma

【Objective】To study the expression of HIF-1 a in endometrial adenocarcinoma and their relationship with tumor angiogenesis,several histopathologic parameters and patient prognosis.In addition,the relationship between HIF-1αand ERαis studied.【Methods】HIF-1α,VEGF,ERαproteins and CD105-MCV were studied immunohistochemically in a group of 56 patients with endometrial adenocarcinoma, 20 patients with normal endometria and 18 patients with atypia hyperplasia.Survival analysis was also studied.【Results】1.In 60.7%of endometrial adenocarcinoma,27.8%of atypia hyperplasia and 45.0%normal endometria,expression of HIF-1a was observed.There was a significant difference between them(P＜0.05).HIF-1a overexpression showed no significant correlation with histopathologic parameters but has an impact on survival of cancer patients(P＜0.05).2.ERa expression was detected in 60.7%of endometrial adenocarcinoma and 77.7%,100%in atypia hyperplasia and normal endometria,respectively.There was a significant difference between them(P＜0.05).High ERa protein expression was associated significantly with the less depth of myometrial invasion(P＜0.05),but it has no impact on survival of cancer patients(P＞0.05).3.The VEGF positive expression rate(89.3%)in endometrial adenocarcinoma was significantly higher than that in atypia hyperplasia(72.2%)and normal endometria(60.0%)(P＜0.05).Increased VEGF expression has no association with clinic pathological parameters and survival time(P＞0.05).4.The CD 105-MVD in normal endometria,atypia hyperplasia and endometrial adenocarcinoma was 21.6±11.5,27.9±13.7 and 47.7±12.8 respectively.The CD 105-MVD in endometrial adenocarcinoma was significantly higher than that in atypia hyperplasia and normal endometrium(P＜0.05)and was significantly correlated with the clinical stage,depth of myometrial invasion.There was a marginal association of high CD105-MVD and a poor prognosis(P=0.0767).5.In the cases of endometrial adenocarcinoma,there was a positive correlation between expression of HIF-1a and ERa(r=0.265,P=0.049),HIF-1a and VEGF(r= 0.355 P=0.007),HIF-1a and CD105-MVD(r=0.282 P=0.035).【Conclusions】1.The up-regulation of HIF-1a is a common event in endometrial adenocarcinoma.HIF-1 a expression is related to increased angiogenesis,and to an unfavorable prognosis in endometrial adenocarcinoma.HIF-1αis considered a likely target for the treatment of endometrial adenocarcinoma.2.The correlation of HIF-1a and ERa protein expression provides a new clue to the mechanism and treatment of ERαon endometrial adenocarcinoma.Inhibiting HIF-1αaction could be an approach for the treatment of E2-dependent diseases of the uterus.Combinatorial treatments that target both ERαand HIF-1αmight be especially effective for the treatment of endometrial adenocarcinoma.