| Objective:To investigate the expression of insulin receptor substrate- 1(IRS-1)and phosphorylation of tyrosine in androgen sterilized rats(ASR) and explore molecular mechanisms of insulin resistance(IR) in ASR。In addition,to investigate the effects of BSHXHYR on the rats and to analyze the possible mechanism.Methods:ASR model was established by means of testosterone injection subcutaneously at the age of 9 days,The rats were randomly divided into BSHXHYR group(group C,n=15) and model group(group B,n=15);the rats in normal group(group A,n=15) were injected with oil at the same time.The rats in group C were treated with BSHXHYR for 30 days, and the rats in group B and A were treated with water for 30 days at the same time. Testosterone(T),insulin(IN) and fasting plasma glucose(FPG) were measured by Radioimmunoassay.Insulin receptor substrate-1(IRS-1)and phosphorylation of tyrosine in adipose tissue of all rats were analysed by immunohistochemical method.Results:The rats weight in group B was higher than that in group C(P＜0.05);The rats weight in group A was lighter than that in group N(P＜0.05).The rate of ovulation in group C was 66.67%.There was significant difference between group C and group B (P＜0.05).Insulin receptor substrate- 1(IRS-1)and phosphorylation of tyrosine in group A were significantly higher than those in group B(P＜0.05),and those in group C had great improved.There was also significant difference between group C and group B (P＜0.05)Conclusions:The decrease of insulin receptor substrate-1(IRS-1)and phosphorylation of tyrosine in adipose tissue of androgen sterilized rats(ASR),which induces impairment of the insulin signal pathway,may be one of the mechanisms leading to insulin resistance. BSHXHYR could increase the tyrosine phosphorylation levels of IRS-1 and phosphorylation of tyrosine in adipose tissue in IR rats,which maybe one of its mechanisms in lowering blood glucose and improving insulin sensitivity of the target tissues. |
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