Optimization Of Extraction Process Of Active Components From High EGCG Tea Tree And The Research On The Anti-senile Function Of The Tea Polyphenol

The fresh leaves of the tea tree variety "1005" with high EGCG content was used as materials, to carry out the research on the following areas: the deactivation of enzymes of fresh leaves, the extraction of tea polyphenol, the separation and purification of tea polyphenol, the purification of EGCG, the elimination free radical ability of tea polyphenol extraction and the anti-senile function in vivo.The results of enzyme deactivation of fresh leaves indicates that the EGCG contents (after enzyme deactivation)from high to low in turn are 11.13% by microwave, 9.58% by drying oven, 9.27% by frying in pan, and 8.44% by stream. The best way for enzyme deactivation is using microwave, as it increased EGCG content by 2.69%, 1.68%, and 1.55% compared to using stream, frying in pan and drying oven, respectively.The results of extraction, the separation and purification of tea polyphenol indicates that the most suitable craft findings to indicate that the best extraction conditions are 50% ethyl alcohol in the solvent, 4 times extraction, 50 mins, 70℃, volume rate 1:50. Compared to D101 and H1020 resin, the DM301 resin is better on the separation and purification of tea polyphenol. The parameters for the separation and purification of tea polyphenol by DM301 are extraction solution PH 2～3, flow speed 2ml/min for adsorption and desorption, 80% acetone in desorption solution. Based on these results, using high EGCG tea tree, Oolong tea and Huangdan as materials, we got three kinds of tea polyphenols, with average extraction rate 22%, and average purity above 95%. The tea polyphenol extraction rate of high EGCG tea tree was 4% and 2% higher than Oolong tea and Huangdan. The EGCG contents in tea polyphenol from high to low in turn are 47.07% in high EGCG tea tree, 31.55% in Oolong tea, 26.08% in Huangdan. EGCG content in tea polyphenol of high EGCG tea tree is 15.52% and 20.99% higher than Oolong tea and Huangdan.The research on separation and purification of EGCG indicates that, with flow speed 0.5ml/min, 60% ethyl alcohol in elution solution and Sephadex LH-20 glucosan gelatin column, the EGCG extraction rates of tea polyphenol from high EGCG tea tree and Huangdan were 33.3% and 16.6%, with both of the purities between 96%～981The results of tea polyphenol elimination free radical ability and the animal in vivo anti-senile research indicated that, compared to the tea polyphenol extracted from normal tea tree, tea polypphenol from high EGCG tea tree had stronger ability to eliminate free radicals, 9.37% higher than those in Huangdan. On the extension of fruit fly's lifetime, the tea polyphenol from high EGCG tea tree was able to extend longer lifetime of fruit fly compared to Huangdan, 14.5% higher (male) and 14.9% higher (female). On the aspects of fruit fly in vivo SOD value and total oxidation resistance value, SOD value for tea polyphenol from high EGCG tea tree is 32.9% (male) and 12.1% (female) higher than Huangdan, and total oxidation resistance values were 44.1% (male) and 28.5% (female) higher. Using different extraction solutions, the results were different on the lifetime extension of fruit fly. Compared to using boiled water, extracted tea polyphenol by ethyl alcohol were 6.51% (male) and 6.45% (female) higher on the average of the fruit fly's lifetime extension. On the aspects of fruit fly in vivo's SOD value and total oxidation resistance value, compared to using boiled water, using ethyl alcohol was better on increasing SOD value and oxidation resistance value, SOD value improved 9.53% (male), 9.43% (female), total oxidation resistance values improved 31.9% (male), 18.4% (female).