| Objective: The study was to investigate the protective effects of Osthole LPD solution on donor lung and possible mechanism in combined heart-lung transplantation.Methods: 24 rabbits were randomly divided into two groups: the control group and the trail group,each group has 12 rabbits.The harvested lung blocks were flushed with and stored in the modified low potasssium dextrone (LPD) solution in control group,but in the trail group LPD containing Osthole injection (20mg/kg) was used.The lung were stored at 4℃for 12h in both groups.After preservation,the isolated rabbit lung reperfusion models were established and the lungs were perfused for 1h.PaO2 and PaCO2 were measured at just beginning time,15min,30min,45min and 60min time during reperfusion.The protective effects was assessed by measuring the concentration of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) and myeloperoxidase (MPO) in the lung tissues.After finishing the observation, the tissue specimens were taken for analysis of wet/dry ratio (W/D). Light microscopic and ultrastructural changes were also examined.Results: The level of PaO2 decreased gradually,while PaCO2 increased gradually during the reperfusion.The difference of PaO2 and PaCO2 in control group and in trail group was obvious after 30min reperfusion (P<0.05), and the difference was greater after 45min and 60min reperfusion (P<0.01). After reperfusion,the value of SOD in trail group increased evidently than in control group (P<0.01). However,the value of MDA,MPO and W/D in trail group decreased evidently than in contral group (P<0.05). The light morphologic and ultrastructual damages in trail group were milder than in control group.Conclusion: The modified LPD solution which containing Osthole injection can protect the lung effectively,decrease the activity of MPO,can help in clearing of oxygen free radical, and inhibit the concentration of PMN,so alleviate the ischemia-reperfusion injury,improve the protective effect of lung preservation.
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