| The gastric carcinoma is one of the highest disease rates of cancer, especially in Asia and Africa. Diffusion and metastasis is the important cause of cancer-related death. In China, at least 160 000 people died of gastric carcinoma, with the highest death rate for all cancer. Though obvious improvement has been achieved in diagnostic and therapeutic research of gastric cancer, most of gastric carcinoma patients were diagnosed as advanced stage. The treatment of appeasable surgery and chemo-radiotherapy did not significantly improve survival time of advanced gastric carcinoma patients. It is a key task for cancer research to search novel prognostic marker, improve early diagnostic rate, and new target of molecular targeted therapy of gastric carcinoma.Objectives :To study the expression and clinical significance of Her-2/neu,TopoIIαin gastric carcinoma tissue,which provided a theoretical basis on the combined therapy to gastric carcinoma.Methods:1. To collect 90 cases of gastric carcinoma and 30 cases of normal gastric tissue from First Hospital Affiliated to Shan Xi Medical University.2. To detect Her-2/neu and TopoIIαexpression in gastric carcinoma and normal gastric tissue by immunohistochemistry assay(PV-9000).3. To statistically analyze the relationship between Her-2/neu and TopoIIαexpression and various clinicopathologic parameters using SPSS 11.5.Results:1. In 90 specimens of gastric cancer,the positive expression rates of Her-2,TopoIIαwere 20.0%(18/90),85.6%(77/90) respectively.In 30 specimens of normal gastric tissue , the positive expression rates of Her-2,TopoIIαwere 3.3%(1/30),60%(18/30) respectively. There are the significant difference between two groups(P<0.05).2. According to Lauren type , gastric carcinoma is divided into intestine type,suffusion type,mixed type. the positive expression rates of Her-2/neu were 31.8%,9.5%,0% respectively, there are the significant difference between three types(P<0.05).It indicates that the over-expression of Her-2/neu has relation to the Lauren type of gastric carcinoma. In well and moderately differentiated,poorly differentiated gastric carcinoma,the positive expression rates of Her-2 were 10.6%,30.2% respectively,difference was significant. It indicates that the over-expression of Her-2/neu has relation to the differentiation of gastric carcinoma.The over-expression of Her-2/neu in gastric carcinoma without lymphatic node metastasis were 2.9%, and that with lymphatic node metastasis were 30.4%, difference was significant. However, the expression of Her-2/neu in gastric carcinoma were not related to age, sex, position, clinical stage, invasion depth.3. The expression of TopoIIαhad related to the differentiation degree of gastric carcinoma, while there was no relationship with age,sex,position,size,clinical stage, invasion depth,Lauren type, lymphatic node metastasis.4. 72.2%(13/18) of Her-2/neu amplification had concomitant TopoIIαamplication. 72.2%(13/18) of Her-2/neu amplification had concomitant TopoIIαamplication. There was no relationship between Her-2/neu and TopoIIα, but their co-expression rate was relatively high.Conclusions:The overexpression of Her-2/neu and TopoIIαmay play a role in the pathogenesis of gastric carcinoma, combined detection would be of benefit in selecting chemotherapeutic agents and evaluating prognosis in gastric carcinoma.
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