Level And Change Of Th1/Th2 Cytokines In Patients With Hepatitis B During Nucleic Acids Antiviral Therapy

Objective: To investigate the significance of serum IL-10,IL-2 and IFN-γlevel changes in Hepatitis B Virus infection patients.Methods: The serum IL-2,IFN-γand IL-10 level of 63 patients who had infected with Hepatitis B, including 21 HBV carriers and 42 chronic HBV patients (21 patients with mild degree,21 patients with moderate or serious degree),were detected by ELISA. 10 healthy human were detected as control group. The Hepatitis B viral infection marker of the patients who received antiviral therapy (Lamivudine and Adefovir dipivoxil were taken in turn) were detected by ELISA before the treatment and each 12 weeks during the therapy. The liver function and the HBV-DNA were also detected at the same time. The serum IL-2,IFN-γand IL-10 level were detected by ELISA before the treatment and after 52 weeks'therapy.Results:1. The serum IL-10 level in patients who had infected with Hepatitis B (including HBV carriers and chronic HBV patients) was obviously higher than control group (P <0.01). Both the HBV carriers group and patients with moderate or serious degree group were obviously higher than mild degree group (P<0.01); The serum IL-2 level in chronic HBV patients (including patients with mild degree and patients with moderate or serious degree) was obviously higher than the HBV carriers group and control group(P<0.01); The serum IFN-γlevel in the patients with moderate or serious degree was obviously higher than the other groups(P<0.01),the HBV carriers group was obviously higher than control group.2. The serum IL-10 level in patients who had infected with Hepatitis B (including negative virus load group and positive virus load group) was obviously higher than control group (P <0.01),the patients with low virus load group was obviously higher than high virus load group (P<0.01),the high virus load group was obviously higher than negative virus load group(P<0.01); The serum IFN-γlevel in patients with high hepatitis B virus load was obviously higher than control group,negative virus load group and low virus load group(P<0.01).3. The serum IL-2/IL-10 ratio in patients (including HBV carriers and chronic HBV patients) was obiviously lower than control group (P<0.01), the patients with mild degree group was obviously lower than moderate or serious degree group (P<0.01), and the carriers group was obviously lower than mild degree group and moderate or serious degree group (P<0.01); The serum IFN-γ/IL-10 ratio in both of the carriers and mild degree group was obviously lower than both of the control and moderate or serious degree group(P<0.01).4. The serum IL-2/IL-10 ratio in patients who had infected with Hepatitis B (including negative virus load group and positive virus load group) was obviously lower than control group (P <0.01),the patients with high virus load group was obviously lower than negative virus load group (P <0.01).The serum IL-2/IL-10 ratio and IFN-γ/IL-10 ratio in the patients with low virus load group were obviously lower than negative virus load group and high virus load group (P <0.01); The serum IFN-γ/IL-10 ratio in the patients with negative virus load group and low virus load group was obviously lower than control group (P <0.01).5. The serum concentration changes of IL-10 correlated with the copies of HBV DNA (P<0.05). The serum concentration changes of IL-2 correlated with level of ALT and AST (P<0.05), and the changes of IFN-γhad obviously correlate with level of ALT and AST (P<0.05).6. The serum IL-2 level of the patients who received antiviral therapy and had a good curative effect was obiviously higher than control group and before the treatment,and higher than the ones who did not have a good curative effect(P<0.05); The serum Il-10 and IFN-γlevel of the patients in all groups were higher than control group(P<0.05); The serum IL-10 level of the patients after the treatment and had a good curative effect was lower than its level before the treatment(P<0.05).7. The serum IL-2/IL-10 ratio of the patients who did not have a good curative effect either before or after the treatment was obiviously lower than control group (P<0.05). The serum IL-2/IL-10 ratio of the patients who received antiviral therapy and had a good curative effect was obiviously higher than before the treatment and the ones did not have a good curative effect (P<0.05), The serum IL-2/IL-10 ratio of the patients who have a good curative effect before the treatment was obiviously lower than control group (P<0.05).Conclusion1.The serum IL-2,IFN-γand IL-10 was at a high level in patients who had infected with Hepatitis B,three of them play a part in the hepatitis .The serum level of Th1/Th2 cytokines changed, which may be one of the reasons of the persistent HBV infection.2.Both of the serum IL-2/IL-10 ratio and IFN-γ/IL-10 ratio reflected the patients who had infected with Hepatitis B had disbalance immune state. The broken balance of Th1/Th2 cells may induce the persistent HBV infection.3. The patients with negative virus load,the chronic patients had stronger immune function than the patients with positive virus load,the carrier patients.Clinical situation of the patients correlated with their immune state.4. The patients who received antiviral therapy (Lamivudine and Adefovir dipivoxil were taken in turn) could retrieve the broken balance of Th1/Th2 cells,the balance of Th1/Th2 cells could predict the effect of antiviral therapy .