An Study Of Neuroprotective Effect Of Leukemia Inhibitory Factor On Mouse Retinal Gonglion Cell After Ischemic-reperfusion Induced Injury

Objective: To observe the expression of Leukemia Inhibitory Factor on mouse retinal gonglion cell and investigate its neuroprotective effect on mouse retinal gonglion cell after ischemic-reperfusion injury.To supply a effected and new method for treating retinal ischemic-reperfusion injury especially glaucoma.Methods: Healthy fifty-four kunming mice(one hundred and eight eyes)were randomly divided into normal control group(eighteen mice,thirty-six eyes), retinal ischemia reperfusion group(RIR group,eighteen mice,thirty-six eyes),and LIF treatment group(eighteen mice,thirty-six eyes) . These three groups were subdivided into group 4 ,8,12 ,18, 24 and 48 hours after reperfusion respectively(every group three mice,six eyes).Transiently elevating the pressure of the anterior chamber with perfusion of normal saline to induce experimental retina ischemia reperfusion injury modle. .After madding mouse models, 0.5ul/g LIF was injected into vitreous cavity of the treatment group mice after ischemia/reperfusion immediately. The eyes were enucleated after reperfusion for 4,8,12,18,24,48 hours.,then fixed, embedded and sectioned.made the sample HE staining and immunofluorescence staining.The morphological change of retina was studied by light microscopy.The number of retinal Gonglion Cell was measured with compute picture analytic system.and statistical conclusions were obtained. The expression of LIF on retinal Gonglion Cell was observed with fluorescence microscope.Result:1. Light microscope: In the ischemia reperfusion group, we can found edema of retinal (especially in the neuron fibrary layer and inner plexiform layer) in the early stage and the retinal atrophy, degeneration and necrosis were found in the later period. We can found that the RGCs was much less than the normal control group in the ischemia reperfusion group. In the treatment group, the RGCs was much more than the ischemia reperfusion group.2. Fluorescence microscope: LIF expressed on retinal gonglion cell in every griup.3. The number of RGCs :Compared with the control group and the retinal ischemia reperfusion group, the number of RGCs had no significant differences (P>0.05) at 4th hour after reperfusion .The number of RGCs is much more (P<0.05)in the LIF treatment group than the ischemia reperfusion group at 4th,8th,12th ,18th, 24th and 48th hour after reperfusion ,but is less (P<0.05)than the control group at 4th,8th,12th ,18th, 24th and 48th hour after reperfusion .Conclusion:1. Pathological changes displayed: the retinal ischemia reperfusion model was made successfully.2. As to mouse, there was the expression of LIF on retinal gonglion cell .3. LIF could provide neuroprotective effect for mouse retinal Gonglion Cell after ischemic-reperfusion induced injury...