Fast Identification Technology Applied In The Infectious Diarrhea

Objective : Because of the wide variety of exotoxin-producing capabilities, Staphylococcus aureus(S. aureus)is an important pathogenic bacteria for humans. Furthermore, staphylococcal infections are of more serious concern for health-care professionals than old days. S. aureus represents one of the bacterial species most frequently encountered in human infections, and many diarrheal diseases can be observed in the hospital environment. The use of broad-spectrum antibiotics and the growing isolation of methecillin resistant S. aureus might make S. aureus enteritis more common, especially in patients with hospital-acquired diarrheal diseases of unknown etiology. The rapid detection of pathogenic bacteria is extremely important in biotechnology and clinical diagnosis. Capillary electrophoresis(CE) has been utilized in the field of bacterial analysis for many years, but to some extent, simultaneous separation and identification of certain microbes from complex samples by CE coupled with ultraviolet (UV) detector is still a challenge.Method:In this paper, we propose a new strategy for rapid separation and identification of S. aureus in bacterial mixtures by means of specific monoclonal antibody coated latex coupled with capillary zone electrophoresis (CZE).Results:An appropriate set of conditions that selectively isolated S. aureus from the microorganisms Escherichia coli, Pseudomonas aeruginosa, and Klebsiella peumoniae were established. S. aureus could be differentiated from the others by unique peaks in the electropherograms. The LOD is as low as 9.0×10~5 CFU/mL. We have also utilized this technology to identify S. aureus in a stool sample coming from a healthy volunteer spiked successfully with S. aureus.Conclusion:This CZE-UV technique can be applied to rapid diagnosis of enteritis caused by S. aureus or other bacterial control-related fields needing rapid identification of target pathogens from microbial mixtures. In theory, this method is suitable for the detection of any bacterium as long as corresponding bacterium-specific antibody coated latex is available.