Human Recombinated Endostatin Combined With Chemical Drugs For The Treatment Of Breast Cancer In Animal Study

Objective: to investigate the inhibitoty effect of Human recombinated Endostatin combined with cis-diamminedichloroplatinum(DDP) on human breast cancer MCF一7 cell strain in athymic mouse in order to supply theoritical evidences for clinical treatment of breast cancer in application of Human recombinated EndostatinMethod: to administer drugs after setting up athymic mouse model with breast cance for different groups and to observe tumor growth condition , 20 days later to take off the tumor with lung and liver ; HE stain was employed to detect matastasis of tumor through; Reverse trancription-polymerase chain reaction and Western blot analysis were used to determine the expression of VEGF at both transcriptional and translational levels; flow cytometry was used to measure apoptosis ratio of cancer cell .Result :1.all the athymic mouse were alive with tumors burden which were oval-shap with smoothy surface at early stage and were iregular-shape with cauliflower-like nodus that were plenty of vessels at later stage.2.In contrast with the gross tumor volumes of cis-diamminedichloroplatinum(DDP) group,Human recombinated Endostatin group and control group,these of DDP plus Endostatin group were decreased significantly(p<0.05).DDP group were deflated in comparision to Endostatin group(p<0.05).Significant differences were existed between different groups(p<0.05) .95%CI of gross tumor volume of DDP plus Endostatin group,DDP group,Endostatin group and control group were (0.6506-0.7113) cm3,(1.1207-1.3565) cm3, (1.5549-2.1591)cm3,(3.0017-3.7544)cm3.And tumor inhibited rate of interfered groups were 92.1%,57.3%,36.5% respectively.3.Lung matastasis rate within groups were 0,5%,20%,90% respectively with statistics differences (p<0.05).4.All the mouses wewe at good condition before experiment and all were alive after it.Through dynamic observation,control group mouses react clumsily with less activites.Positive control group mouses were obvious maransis.Interfered group mouses forage,drink and act normally without diarrhea and congestion.5.The mRNA of VEGF and GAPDH were expressd in both control group and interfered group,whereas the expression of VEGF in ODD plus Endostatin group was lower than that in control group.There was significant statistics difference within different groups.6.The protein expression of VEGF in DDP plus Endostatin group was lower than that in control group, There was significant statistics difference within different groups.7.The mean rate of apoptosis in DDP plus Endostatin group was 31.6% which was higher than 15.7% in control group. There was significant statistics difference within different groups.Conclusion:1. Endostatin can inhibit growth of breast cancer without obvious toxic side effect , whereas the effect of controling growth velocity on cancer cells is not as good as DDP.2. Endostatin possess the inhibitory action on matastasis of athymic mouse breast cancer.3. Endostatin combined with DDP can improve living condition and extend life span of the athymic mouse by enhancing its therapeutic effect on breast cancer meanwhile cutting down its side effect.