| Objective:Acute cerebral ischemic stroke is clinical common disease and frequently encountered disease ,which is principal disease that threaten people's health seriously.Stroke often occur on middle-aged and old-aged people. Because their entrails function decline gradually, adding their living standard is raising,they eat and drink without temperance ,finally all of these hurt their appetite, asthenic yin causeing predominant yang, dys-plenism, stagnation of phlegm converting into heat ,accompanying hepatic wind across veins and arteries,stagnant blood causing essential resisitance,muscle and vessel losing nutrition or hepatic wind with pyrophlegm upper punch brain,QI and blood inverse,bleeding resulting from adverse flow of QI, covering the upper orifices.So we can cure the sroke by suppressing this process.Danxing tongluo decoction(DXTLD) is a mixture of some herbs, such as Danshen, Dannanxing, Huaihua, Sanqi, Huangqi, etc. It has functions of cleaning away stagnated blood and heat, restoring normal functioning of the body to consolidate the constitution and resolving mass. Danshen, Dannanxing are the chief herbs of the dose, which can promote blood flow, deoppilate meridian, clean heat, eliminate sputum, etc. Huaihua, Sanqi, Chuanxiong, Honghua and Dahuang, are the secondary herbs of DXTLD. So the dose can efficaciously preclude the sputum stagnated blood and sputum blockaging in brain meridian. On account of appearance and substance are both to be cured, we can get a fine therapeutic effect by using it to cure the stroke.Some research discover that natural extractive of Dannanxing can suppress platelet aggregation which caused by ADP and collogen,also can relax blood vessel visible and reduce vascular resistance. Chuanxiong can permeate blood-brain barrier ,relax blood vessel,improve cerebral ciculation and haemodynamics,resisit oxidation.The active constituent of Denshen is salviol.It can relax blood vessel and improve microcirculatory.It also can eliminate oxygen free radical directly,decrease consumption of peroxide dismutase,raise activity,then brain cell can use more oxygen.Combining tradicional Chinese medicine and western medicine can get a fine therapeutic effect.People gradually pay attention to the function of apoptosis on cerebral ischemic stroke. Cellula nervosa apoptosis takes part in the delayed neuronal death which can coursed by both acute focal cerebral ischemia and transient cerebral ischemia.But apoptosis makes significant effect on delayed neuronal death and the semidarkness zonal .There so much control factor on the process of cellula nervosa apoptosis,which can prevent cellular necrosis from apoptosis.It provides a new way to cure cerebral ischemic stroke.So using medicine stop cellula nervosa apoptosis is a hot spot aroud the world.There are all kinds of apoptotic profilin,but the new one is inhibitor of apoptosis protein(IAPs),which own special molecular constitution and the function that suppress apoptosis.IAPs is concerned with cellula nervosa apoptosis and tumor.As the target protein,IAPs maybe become the new method to cure the stroke.Methods: Fifty healthy male Wistar rats were randomly assigned to 5 groups, 10 rats per group: sham operation group , ischemia/reperfusion (I/R) group, DXTLD high dosage group , DXTLD low dosage group and Nimodipine(NIMO) group. DXTLD groups were given to DXTLD at dose of 1.5ml/100g,0.75ml/100g;NIMO group at 2ml/100g,once a day. Saline were given to sham operation group and I/R group at 2ml/100g weight. After 7-days,the rats of all the groups except sham operation group were made into cerebral ischemia reperfusion injury models by thread embolism method. All the rats were killed and their brains were taken out after 1h ischemia and 24h reperfusion injury. The Caspase-3 expression and Survivin expression in brain tissue were measured with immune- ohistochemical method .Results:1.The ultrathin sections of brain tissue is made research about histomorphology by the common HE tacho-staining technique after rat cerebral ischemia and reperfusion:The group of sham operation group has normal cellular structure;The margin of focus of infection comparatively is distinct and the degree of cellular necrosis is more serious in the group of I/R group. The cells are obviously cutaneous dropsy. At the center of ischemic area we can find cellula nervosa necrosis, circum-cell diastem become broader, nucleus shrinkages, caryotheca fragments, chromato- spherite disappears, etc; While in the group of DXTLD high dosage group and NIMO group we can get the outcome that neuronal degeneration fairly light at ischemic region. Besides these, cells comparatively regular and full, and there are integrate living cells at the around of semidarkness area.2. to observe the expression level of Caspase-3 gene and Survivin gene by the ultrathin sections of brain tissue and the method of immunohistochemistry technology : After the five groups rats had the medicine or substitute medicine and then disparity has significantly disparity between DXTLD high dosage group and I/R group(P<0.05). Videlicet : DXTLD could obviously increase the Survivin expression, decrease the Caspase-3 expression in cerebral cortex and hippocampus and relieve nerve cell injury.3.Serumal LDH level of ischemia/reperfusion group were increased significantly compared with sham-operation group (P<0.05); Serumal LDH level of the treatment group were increased compared with the sham- operation group, but it decreased remarkably compared with the ischemia/ reperfusion group (P<0.05).Conclusions: DXTLD can protect brain tissue of cerebral ischemia reperfusion injury and improve the nerve function. The mechanism may be related with the increasing of Survivin expression a decreasing of Caspase-3 expression.
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