Experimental Studies On The Injury Of Intestinal Barrier Function Induced By Carbon Dioxide Pneumoperitoneum

Objective: To explore the influence on intestinal barrier function induced by the different intraabdominal pressure of carbon dioxide pneumoperitoneum .Mothod: SD rats model of pneumoperitoneum intraabdominal hypertension were established by insufflating carbon dioxide into abdominal cavity for 90min.45 SD rats(15 in each group)randomly were divided into control group(0 mmHg) which was not insufflate carbon dioxide,10mmHg group and 20mmHg group. Again each pneumoperitoneum group was divided into three groups at 5min,60min,120min after deflation(5 in each group),and correspondingly control group was erected. Intestinal mucosa malondialdehyde(MDA), nitric oxide(NO),Glutathione(GSH) and the blood diamine oxidase (DAO) were measured,and pathologic examinations of the intestine were observed by optical microscope.Result:1. MDA values in the 20mmHg group were higher than in other two groups significantly at 5min,60min,120min after deflation(P<0.05). There was a significant increase of MDA concentration in the 10 mmHg group only at 5min than control group(P<0.05). 2 .NO values in the 20mmHg group were higher than in other two groups significantly at 5min,60min,120min after deflation (P<0.05). NO values in the 10 mmHg group was higher than in control group,but the difference between the two groups was not statistically significant (P>0.05).3.Both 10 mmHg group and 20mmHg group GSH values decreased at 5 min,60min, 120min after deflation. GSH values in the 20mmHg groups is lower significantly than in other two groups at 5 min, 60min, 120min after deflation(P<0.05). There was a significant decrease of GSH values in the 10 mmHg group only at 5min than in control group(P<0.05). 4. The plasma DAO activity in 20mmHg group was higher than in other two groups significantly (p<0.05),however the plasma DAO activity in the 10 mmHg group is similar to that of control group (P >0.05). 5. There was edema in intestinal mucosa and some epithelial cells slough from villi in 20 mmHg groups,and there was no obvious pathologic change in control groups and 10mmHg groups.Conclusion: 1.10mmHg and 20mmHg carbon dioxide pneumoperitoneum pressure can lead to increase of malondialdehyde(MDA) values and nitric oxide (NO) values,decrease of Glutathione(GSH)values in intestinal mucosa ,and the increase of the intraabdominal pressure aggravate oxidative stress in intestinal mucosa.2. 20mmHg Carbon dioxide pneumoperitoneum can increase DAO activity in the blood and injury intestinal barrier function; however 10mmHg Carbon dioxide pneumoperitoneum can not influence the blood DAO activity and intestinal barrier function.3.Oxidative stress by carbon dioxide pneumoperitoneum could be one of the mechanisms of intestinal barrier function injury.