Study On The Extraction, Isolation, Stracture Analysis And Biological Activity Of Saponins In Dioscorea Zingberensis

Background of the researchOncomelania hupensis is the only intermediate host of schistosoma japonicum Katsumade. So killing snail is one of the critical technologies to controling schistosomiasis . Now,chemical molluscicide such as niclosamide which is used commonly is high effective.but it is expensive and toxic to fish.therefore,from the 1930s,studying efficient,safe and inexpensive plant molluscicide become the hot spot in the world. But now there is no one plant molluscicide which can reach WHO'S standards.Content and significance of the researchIt is to study on exteraction and isolation,structural analysis and biological activity of sponins,which is from molluscicidal plant Dioscorea Zingberensis,For creation of new molluscicide offering basis for designand material basis.Methods and results of reaserchExtration and isolation and structural analysis of sponins from DZ. The DZ powder was extracted amd speparated by ultrasomit, centrifugal and acctivated carbon decolonzation, and was purificated by r-crystallization,TLC and CCL. Then,we got effective saponins DZ-I.By detection of HPLC VWD and ELSD, the purity of DZ-I is 98%.Stracture analysis of DZ-I is doing.Molluscicide activity and inhibiting GPT activity of DZ-I. Molluscicidal test method according to the WHO'S standards,molluscicidal rates was 91.6 and 100% at 8.0 and 16.0 mg·L^-1(?) of DZ-I for immersion in 48h.The GPT was spearated from snail, under the optimal conditions on the GPT response (0.1mg.ml^-1GPT,5μmol·L^-1 substrate ,Ph6.5, 35℃,30min) by L₂₅(5⁶) orthogonal matrix method,we studied the influence of DZ-I to GPT activity. The induced activity was 168, 138, 96, 75 and 57% at 1.0, 5.0, 10, 50 and 100 mg.L^-1 of DZ-I. The result indicate that the induced activity is highest at the lowest concertration of DZ-I.By the use of flat-panel antibacterial law, the inhibiting rate of 50 mg·L^-1DZ-I is 26.32,1.26,17.39,3.70,28.30 and 4.55% to Fusarium oxysporium , Rhizoctonia solani, Botrytis cinereapers , Gibberella zeae , Dothiorella gregaria , Colletotrichum gossypii respectively in 48 h.