Study On Estrogenic Effect Of Genistein On MCF-7 Cell

Objective: To determine the appropriate culture condition, drug usage and time point in vitro test for MCF-7 cells proliferation test and explore the estrogenic effect and possible mechanism of genistein on estrogen-dependent human breast cell MCF-7 at a low level of estrogen environment.Methods: MTT assay was used to analyze the cell growth curve, estrogen sensitivity of two different kinds of MCF-7 cells and the cell proliferation effect in different culture conditions.The proliferation effect of genistein on MCF-7 cells was analyzed by MTT assay, flow cytometry and Annexin V/PI assay were used to analyze the cell cycle distribution and apoptosis, the method of indirect immunofluorescence assay was used to detect the expression of Estrogen-Regulated protein (PS2), genistein and estrogen antanogist ICI-182780 were used together to detect the effect of MCF-7 cell growth.Results: Compare to the human breast cancer cell of MCF-7 B, cell A was more sensitive toward the estrogen, estrogen had the most sensitive stimulating effect on cell proliferation at the concentration of 10-9 mol/L, and the phenolsulfonphthalein in DMEM and endogenous estrogen in Newborn Calf Serum had stimulating effect on the cell proliferation of MCF-7 cells. Compared with control group, the cell proliferation, DNA synthesis and the expression of PS2 protein of MCF-7 cell were markedly enhanced by genistein with the concentration of 5×10^-7-10^-5 mol/L, however, the effect of apoptosis inhibition was not obvious. On the other hand, the stimulating effect of genistein on MCF-7 cells can be blocked by estrogen antanogist ICI-182780.Conclusion: Different kinds of MCF-7 cells have different sensitivity toward estrogen, MCF-7 cells proliferation test can be influenced by different culture conditions. Genistein enhances cell proliferation, DNA synthesis and the expression of PS₂ protein of human breast cancer cell MCF-7 at a low level of estrogen environment, which may demonstrate an estrogenic effect, and the stimulation effect of estrogen maybe conducted by Estrogen Receptor (ER).