A Study Of The Quantity And Biological Function Of Circulating Plasmacytoid Dendritic Cell In Type 1 Diabetes Mellitus

Objective: To investigate the quantity of circulating plasmacytoid dendritic cell(PDC) in type 1 diabetes mellitus as well as analyze the changes of their biological function.Methods: The ratios and quantities of circulating plasmacytoid dendritic cell in 22 T1DM patients and 20 healthy individuals were investigated by flow cytometry. The biological function was estimated by the yield of IFN-αwhich was produced by the PBMCs stimulated and co-cultured with CpG ODN-2216.Results: The circulating plasmacytoid dendritic cells are numerically decreased (p<0.01) and subsequently produce less IFN-α(p<0.05) in type 1 diabete mellitus versus normal individuals. And PDC count was positively correlated with the yield of stimulated IFN-a in type 1 diabetes mellitus(r=0.439,p<0.05).Conclusion: The numerical decrease and functional changes of the peripheral blood plasmacytoid dendritic cell may play important roles in the immunologic derangement in T1DM. The less yield of IFN-αwhich was produced by the PBMCs may be contributed to the susceptivity of the infective diseases.