| ObjectiveThe development of various combinations of immunosuppressive therapy has significantly improved the short-term graft survival rates and reduced the acute allograft rejection rate. However, a constant proportion of organs were lost every year due to chronic allograft rejection and the toxic side effect of immunosuppressive drugs. Hence, how to induce donor-specific tolerance,prevent acute and chronic allograft rejection,diminish the toxic side effect of immunosuppressive drugs and reduce the drug-using time may be resolving this problem.During pregnancy, the maternal immune system has to tolerate the persistence of paternal alloantigens without affecting anti-infectious immune responsiveness. Despite years of research in this area, the cellular and molecular mechanisms enabling the maternal immune system to support its semiallogeneic fetus are still poorly understood. Since normally the maternal immune system does not reject its semiallogeneic concept, pregnancy has been thought to be a state of immunological tolerance.The aim of present study was used PBMC from pregnant and non pregnant mice and spleen cells from paternal mice to evaluate the influence of pregnancy to the maternal cell-mediated immunology with the technology named mix lymphocyte reaction. We next transplanted skin allograft from paternal mice at early pregnant mice to observe weather pregnancy acts as protect effect on allograft. Then we evaluate the different expression of the protective molecule HO-1mRNA in spleen and uterus between pregnant and non-pregnant mice to discuss the possible protective mechanism.MathodsWe bought 54 female C57BL/6 mice and 24 male BALB/c clean animals. We made skin transplantation model in 6 C57BL/6 mice. 48 female C57BL/6 mice were randomly divided into cell reaction group and skin transplant group and each group were divided into pregnant group and non-pregnant control group. Pregnant group were mated with BALB/c male mice. Cell reaction group mice were sacrificed at day 14 after pregnant, PBMC and spleen cells were collected from spleen of female and male mice to do mix lymphocyte reaction, and use stimulating index to evaluate the intensity of cytoreaction. Also 100 mg of the spleen and uterus were collected and washed with sterile PBS, and then stored in—80℃refrigerator. We used RT-PCR and calculate Ct to evaluate the difference of HO-1mRNA expression in spleen and uterus between pregnant and non-pregnant mice. Skin transplantation of female mice donated by BALB/c male mice were taken place at day 6 after pregnancy and use mean survival time to make comparison. We use SPSS15.0 to analysis.ResultsThe difference of stimulating index between pregnance and non-pregnance mice PBMC mixed with spleen cells of BALB/c male mice were no statistical differences.( P>0.05). In homogenetic transplantation the MST of the skin were over 30 days. The skin allograft mean survival time of pregnant mice showed no longer than non-pregnant mice. We next calculate the HO-1mRNA expression difference. We could find out compared with the elevated HO-1mRNA expression in spleen after pregnancy ,the change in uterus were higher( P>0.05).Conclusion1. The maternal cellular immunity of pregnant group was not suppressed compared with non-pregnant group.2. The skin transplantation taken place in early pregnant mice showed no longer survival time than control group.3. The expression of protective cytokine HO-1mRNA in spleen and uterus wereboth elevated in pregnant group compared with non-pregnant group, and inuterus it showed a significantly greater change.
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