| BeckgroundThe tendon adhesion post-operation in the sheathing canal area is a common and tough problem after tendon injury and there is no effective resolvent so far. tendon adhesion is proliferation and intrusion of periphery tissue during the process of wound healing, and induces the motor dysfunction of tendon. The therapeutic efficacy of clinical treatments of suppressing tendon adhesion is not significant.Nowadays the transforming growth factor TGFβhas received much more schola -stic attention. TGFβis a 25 000 homodimer, and has various functions includeing promoting the aggregation of fibroblast and macrophage, enhancing the angiogenesis, stimulating the production of collagenous, down-regulating the matrix metalloprotein -ase and up-regulating the matrix metalloproteinase inhibitor. TGFβis a important cytokine in acute inflammation and wound healing and the over express -ion can induce the scar hyperplasy and fibrosis[1-2]. It is confirmed that inhibiting the biologi -cal activity of TGFβ1 around the tendon by TGFβ1 inhibitor become a hot spot.In our experiments we studied the possibilities of preventing the tendon adhesion and the influence on tendon healing of TGFβ1 antibody. Concerning about the fast region metabolism and low effectiveness of TGFβ1, the compound of transforming growth factor-β1 antibody and fibrin glue (TGFβ1 Ab + FG) was investigated in order to delaying TGFβ1 antibody release and find a therapeutic way to solve the tendon adhesion.Purpose1. To explore the preventing influences of the compound of transforming growth factor-β1 antibody and fibrin glue (TGFβ1 Ab + FG) on prevention of peritendinous adhesive formation.2. To study the effects of applying the compound of TGFβ1 Ab + FG in region on the function o f healed tendon .MethodsSeventy-two Leghorn chickens were randomly divided into 4 groups (group s A , B ,C and D) , 18 chickens for each group, and the long flexor tendons of the 3rd and 4th toes in zoneⅡof 72 leghorn chickens were transversed and sutured with The 4-strand Cruciate repair technique to make defect models. In group A , TGFβ1 antibody was applied at repair site.In group B, FG was applied at repair site. In group C, The TGF-β1Ab and FG 0.2 ml compound was applied at repair site. In group D, normal sodium was applied at repair site. At 1, 3 and 8 weeks after operation, the chickens tendons in each group were harvested.The first part of our study was to observe the morphological influences of the compound of TGFβ1 Ab + FG on tendon repair. After 1, 3 and 8 weeks post operation, the tendons of 6 chickens in each group were harvested. And the 4th toes were made into frozen section for morphological and histological evaluation. The second part of our study was to detect the biomechanical influences of the compound of TGFβ1 Ab + FG on tendon repair. After 1, 3 and 8 weeks post operation, the tendons of 6 chickens in each group were harvested. And the 3rd toes were obtained for biomechanical test including the gliding excursion,the simulated active flexion and the work of flexion. After all the tests above ,the specimens of each group were made into the compound of phalanx ungula and flexor digitorum profundus muscle to test the tendon anastomosis breaking strength.ResultsFirstly, the results of morphological observation.1. The gross observation showed that the differences of grading of tendon adhesion were not significant among groups A , B, and D(P > 0. 05), but in group C, the differences were significant between A , B, and D after 3 and 8 weeks postoperation (P < 0. 05).2. Histological observation showed that collagen fibers of group A, B and D were in a great disorder, but were well-formed in group C.Secondly, the results of biomechanical tests.1. The gliding excursion ratio of the tendons in group A , B, C and D were 0.45±0.05,0.40±0.10,0.79±0.09,0.25±0.07 respectively after 3 weeks postoperation; the same ratio of the tendons were 0.45±0.07,0.43±0.08,0.80±0.09,0.29±0.05 respectively after 8 weeks. Compared with other three groups, the group C had significant differences after 3 and 8 weeks (P < 0. 05).2. The simulated active flexion ratio were 0.61±0.02,0.67±0.03,0.91±0.03,0.53±0.04 respectively after 3 weeks postoperation; the same ratio of the tendons were 0.61±0.02,0.63±0.03,0.92±0.03,0.53±0.03 respectively after 8 weeks. Compared with other three groups, the group C had significant differences after 3 and 8 weeks (P < 0. 05).3. The work of flexion were18.0±0.77D/N,17.8±1.13D/N,27.6±1.73D/N,15.6±1.27 D/N respectively after 3 weeks postoperation, and were 18.3±0.84D/N,15.3±0.75 D/N,27.9±1.24 D/N,18.6±0.80D/N respectively after 8 weeks. Compared with other three groups, the group C had significant differences after 3 and 8 weeks (P < 0. 05).4. Tendon anastomosis breaking strength were14.2±1.9N,15.2±2.2N,16.0±2.2N, 14.7±2.7N respectively after 3 weeks postoperation, and were 51.9±3.0N, 51.4±1.44 N, 53.3±1.3N, 52.3±2.2N respectively after 8 weeks. There were no significant differences among the 4 groups after 3 and 8 weeks (P > 0.05).Conclusion1. The compound of TGFβ1 antibody with FG could significantly prohibit the formation of fibrous adhesions .2. Applying the compound of TGFβ1 antibody with FG after tendon injury did not interfere with the healing process.
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