Epidemiology Of Blood Borne Viruses Infections In Blood Donors From Jinan Region And Construction Of The Recombinant Prokaryotic Expression Of Human Herpesvirus 8

PARTâ… Epidemiology of blood borne viruses infections in voluntary blood donors from Jinan regionObjective To detect HBsAg,anti-HCV,anti-HIV,anti-HHV-8 in voluntary blood donors from Jinan region,and to analyze the epidemiological properties of the infections of the aboved blood borne viruses.To provide scientific basis for blood transfusion safety and the recruitment of low risk blood donors.Methods1 Serological test43904 blood plasma samples were tested for HBsAg,anti-HCV,anti-HIV by ELISA.The samples that were detected to be HIV positive were sent to Shandong Center For Disease Control And Prevention and were confirmed by Western blotting.520 blood plasma samples were tested for HHV-8 IgG antibody by Kd.1 peptide-ELISk.2 Statistical analysisData were analyzed using SPSS10.0 for windows.Chi-square test were used.Results1.Seroprevalence of HBsAg,anti-HCV,anti-HIV among 43904 blood plasma samples were 2.04%(898/43904),0.78%(344/43904),0.0045%(2/43904); Seroprevalence of anti-HHV-8 among 520 blood plasma samples was 5.962% (31/520).2.No correlation were found between age and HBsAg,anti-HCV seroprevalence(P> 0.05);both HBsAg and anti-HCV seropositivity were significantly associated with occupation(P< 0.05);HBsAg seropositivity was associated with sex.No Significant differences were observed in HHV-8 seroprevalence between sex and age groups.ConclusionsThere were certain HCV,HIV seroprevalence and there were relatively high HBV,HHV-8 seroprevalence in voluntary blood donors from Jinan region.It is necessary to improve the accuracy of detection methods for blood screenings and lightened up the education of blood donor and enhanced Dre-tests before donation.PARTâ…¡Construction of the recombinant prokaryotic expression vector of ORF73C gene of human herpesvirus 8ObjectiveTo construct the prokaryotic recombinant expression vector of HHV-8 ORF73C and lay foundations for development of efficient serological diagnosis kit.MethodsA pair PCR primers of HHV-8 ORF73C gene was designed according to the sequence in genebank and correlative literature,which was engineered the cut sites of Bam HI and Hindâ…¢on the 5' ends to facilitate directional cloning.HHV-8 ORF73C gene was amplified by PCR taking the recombinant plasmid pcDNAHis.LANA1 which including HHV-8 ORF73C gene as template.PCR product was inserted into clone vector of pGEM T -easy and then was inserted into the vector pET30(a+)to construct expressing recombinant plasmid pETORF73C.Results1.Agarose gel electrophoresis analysis showed that the sequences of HHV-8 ORF73C amplified by PCR were correct.2.Sequencing showed that the prokaryotic expression vectors of HHV-8 ORF73C were successfully constructed.ConclusionsA reeombined plasmid vector pETORF73C with a epitope of HHV-8 ORF73C has been successfully constructed.The experiment will establish the basis for expression of HHV-8 ORF73C.