Expressiion Of TGF-β1,bFGF In Cornea Of Fetus With Immunohistochemistry

[Purpose]To evaluate the expression and distribution of transforming growth factor-β1(TGF-β1)and basic fibroblast growth factor(bFGF)in human fetal corneas of different pregnant weeks,and to analysis the difference between these two factors.To give datas in the development of human fetal cornea.[Methods]Fourty-six human fetal eyes ranging in age from 11 to 20 pregnant weeks were carefully extracted within 30 minutes of death.Immunohisto chemistry streptavdin-biotin-complex(SABC)method was employed to detect the expression and distribution of TGF-β1 and bFGF in corneas of human fetuses.Then the correlation between TGF-β1 and bFGF was analyzed.[Results]1.TGF-β1 and bFGF were both expressed in the corneal epithelial cells with different pregnant weeks.2.TGF-β1 and bFGF were both not expressed in corneal endothelum and stroma with different pregnant weeks.3.The expression level of TGF-β1 and bFGF were decreasing with pregnant weeks(P＜0.05).4.The expressional level of TGF-β1 was higher than the expressional level of bFGF in the corneal epithelial cells at the same pregnant week(P＜0.05).5.The positive correlation had been found between TGF-β1 and bFGF(r=0.8037)(P＜0.05).[Conclusions]1.TGF-β1 and bFGF were both expressed in the corneal epithelial cells with different pregnant weeks,which suggests TGF-β1 and bFGF may have some effect in the development of human corneal epithelium.2.TGF-β1 and bFGF were both not expressed in corneal endothelum and stroma with different pregnant weeks.3.The expression level of TGF-β1 and bFGF were both decreasing with pregnant weeks.4.The expressional level of TGF-β1 was higher than the expressional level of bFGF in the corneal epithelial cells at the same pregnant week.5.TGF-β1 and bFGF have a positive correlation in the development of human corneal epithelium.