Antioxidant On Myocardium Oxidative Stress And The Expression Of P22phox In NO-deficient Rats

ObjectTo observe antioxidant VitaminE and Ebselen on myocardium oxidative stress and the expression of p22phox in NO- deficient Rats .Methods40 Wistar rats were divided randomly into 5 groups (n=8): Normal group(C); Experimental control group(L); Ebs group(S) Preventative ; VitE group(V) ; Ebs and VitE group (D), administered by gavage method. Their weight and systolic blood pressure (SBP) were measured fortnightly. The rats were killed after 8 weeks. homogenate of myocardium were collected for biochemical examination such as NO, AngII,MDA and O2-. Measure the ratio of heart weight- body weight (HW/BW). The expression of p22phox protein in myocardium were measured by Western blotting.Results①SBP of the rats increased gradually .After 8 weeks, SBP of all rats in experimental groups was obviously higher compared with that of C (P<0.01), and SBP of the antioxidant teams was lower than that of L (P<0.05).. SBP of D was obviously lower than that of S (p<0.01), and SBP of S was fairly lower than that of V (P<0.05).②The NO level, in the tissue of heart of each experimental group were obviously lower than those in C, and those in the antioxidant groups were predominantly higher than those in L(p<0.05), the combination groups more higher (p<0.05).And those in Ebselen groups were higher than those in VitE groups (p<0.05).③T he level of AngⅡ,MDA ,O2- in the tissue of heart,the ratio of heart weight-body weight (HW/BW) of each experimental group were obviously increased than C (p<0.05) , the combination administration group increased less than the single administration groups, and Ebselen groups increased less than VitE groups (p<0.05).④the ratio of heart weight-body weight (HW/BW) of each experimental group were obviously increased than C (p<0.05) , the combination administration group increased less than the single administration groups, and Ebselen groups increased less than VitE groups (p<0.05).⑤the expression of p22phox of each experimental group were obviously increased than C (p<0.05) , the combination administration group increased less than the single administration groups, and Ebselen groups increased less than VitE groups (p<0.05).ConclusionEbselen and VitE can decreas the cardiac oxidative stress and the expression of p22phox in NO- deficient Rats .Ebselen is better than VitE, and the combination is better than single used;...