The Expression And Significance Of CARD11, Bcl-2 And NF-κB In Diffuse Large B-cell Lymphoma

Objective: To study the expression and significance of CARD11,Bcl-2,NF-κB in the common immunology types of diffuse large B-cell lymphoma and explore the correlativity during them.Materials and Methods: 60 specimens of diffuse large B-cell lymphoma patients and 20 specimens of reactive hyperplasia lymph node were selected, CARD11,Bcl-2 and NF-κB were studied by using tissue chip technique and the MaxVision immunohistochemical methods.Results:①The positive rates of CD10,Bcl-6,MUM1 and CD138 in 60 specimens of diffuse large B-cell lymphoma patients were 30.0%,58.3% ,58.3% and 1.7% respectively.②In 60 specimens of diffuse large B-cell lymphoma patients. immunology type: GCB 25 cases, ABC35 cases. 14 cases had the profile CD10(+)/bcl-6(+) and 11 cases had the profileCD10(-)/Bcl-6(+) , 21 cases had the profile CD10(-)/Bcl-6(-), 13 cases had the profile CD10(-) /bcl-6(+)/MUM1(+)/CD138(-) and 1 case had the profile CD10(-)/Bcl-6(+)/MUM1 (+) /CD138(+).③In 60 specimens of diffuse large B-cell lymphoma patients. Immunology type: GCB 25 cases, ABC 35 cases. GCB 25 cases, the positive rates of CARD 11was 24.0% ,ABC 35 cases, the positive rates of CARD11 was57.4%, there being significant difference between the two groups(P<0.05); GCB 25 cases, the positive rates of Bcl-2 was32.0% ,ABC35 cases, the positive rates of Bcl-2 was 62.9%, there being significant difference between the two groups(P<0.05); GCB 25 cases, the positive rates of NF-κB was 28.0% ,ABC 35 cases, the positive rates of NF-κB was 68.6%, there being significant difference between the two groups(P<0.05); The positive rates of Bcl-2 in CARD11 positive and native specimens were 80.8%,26.5% respectively, there being significant difference between the two groups( P<0.05). The positive rates of Bcl-2 in NF-κB positive and native specimens were 58.1%,41.0% respectively, there being significant difference between the two groups( P<0.05). The positive rates of NF-κB in CARD11 positive and native specimens were 76.9%,32.4% respectively, there being significant difference between the two groups( P<0.05).Conclusion: 1,The high expression of CARD11,NF-κB and bcl-2 were related to ABC immunology type of diffuse large B-cell lymphoma ; 2,There is significant positive correlation between CARD11 and Bcl-2, CARD11 and NF-κB, Bcl-2 and NF-κB , respectively.