Empirical Study Of Hyaluronidase On Traumatic Vitreous Hemorrhage's Turnover

Objective: Through establishing the experimemental animol model of traumatic vitreous hemorrhage,investigate the effect of Hyaluronidase on traumatic vitreous hemorrhage's turnover ,and the possibility of induce complete posterior detachment of vitreous ,to offer experiment data of cut the hematocele vitreous body easily in vitrectomy.Methods: There is twenty eight pigmented rabbits with fifty-six eyes. All the left eyes are blank control.All the right eyes were posed the model of traumatic vitreous hemorrhage,fourteen rabbits with fouteen eyes were choosed at random received Hyaluronidase 20IU/0.1ml injected in vitreous space, and the others were injected with equivalent dose of balanced solt as control after one day. All the rabbits were monitored by clinical examination ,intraocular pressure and eyeground photograph at one day,three days,seven days,fourteen days,twenty-one days and twenty eight days.Then two rabbits in each group was sacrified ,measure the basic fibroblast growth factor by draw off vitreous humor, Light microscopy and scan electron microscopy examination were performed for the histological changes of retina .Result: (1) The grade of vitreous opacity were no obvious change in experimental group and positive control group at one day,three days,seven days, vitreous opacity of experimental group was lower than positive control group after fourteen days,there were significantly difference in two groups. (2)There were no obvious change of IOP in each groups at six hours before inject drug. There were significantly difference of IOP between experimental group and positive control group, blank control group at at one day,three days,seven days. There were no obvious change of IOP in each groups after seven days.(3) The density of bFGF were increase in experimental group and positive control group with the lapse of time,bue the positive control group riser than experimental group,at fourteen days The density of bFGFwere1.24 pg/ml in positive control group , it's only 0.62 pg/ml in experimental group, it's no obvious change in blank control group.(4) The amount of fibroblast on the hyperplastic membrane in experimental group not so many as positive control group,there were significantly difference in two groups after seven days.(5)There were retinal edema and balloon change of ganglion cell ,thickening of inner limiting membrane in Light microscopy between experimental group and positive control group; but the hyperplastic membrane in experimental group not fewer positive control group.(6) The part of posterior vitreous cortex and internal limiting membrance connection tight at one day to twenty eight days,no produce in complete posterior detachment of vitreous. Conclusion: No retinal toxicity of Hyaluronidase 20IU/0.1ml injected in rabbit's vitreous space, accelerate the disappear of vitreous hemorrhage,then delay the shape and the development of proliferative vitreous retinopathy.It can make intraocular pressure temporal degrade,bue can't result in complete posterior detachment of vitreous.