| Objective In this study we used amiodarone and irbesartan before reperfusion to interfere in myocardial ischemia-reperfusion injury in rat heart and investigated the preventive and therapeutic effect of amiodarone , irbesartan and both combination on myocardial ischemia-reperfusion injury in rat hearts .Methods 50 Sprague-Dawley male rats are divided into five groups randomly,sham group,ischemia-reperfusion group,amiodarone group (2.5mg/kg.d) ,irbesartan group (30mg/kg.d) , combination of Amiodarone and Irbesartan group. After 1 week of pretreatment, the left anterior coronary descending artery was ligated in 30min,then the ligateing was relieved 120min with hemodynamic and electrocardiographic monitoring.The ECG changes were recorded incessantly from myocardial ischemia to reperfusion and the left ventricle hemodynamic parameters were recorded in the end. The ECG was analysed and given score in LamBeth regulation. Myocardial tissue of infarcted zone was scissored to measure MDA content and SOD activity. The tissue sections were stained by N-BT and weighed.Results The scores of ischemia reperfusion arrhythemias(VP,VT or VF) , hemodynamic variables ,myocardiol relative infarct weight, the levels of tissue SOD activity and MDA content,myocardial infarction size were significant different among sham group, ischemia reperfusion group and drug intervention group (P<0.05),and were different between groups Amiodarone,Irbesartan and combination of Amiodarone and Irbesartan(P<0.05) too,but were no significant different between Amiodarone group and Irbesartan group(Pï¹¥0.05).Conclusion Amiodarone and Irbesartan have significant prevention effects on rat model of myocardium ischemia-reperfusion injury.There is additive effect when the two drugs were used in combination. |
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