Research Of Endothelization On Titanium Oxide Film Surface Modification Via Immobilization Of Laminin And Fibronectin

In this work,titanium oxide(Ti-O)films were synthesized using Unbalance Magnetron Sputtering(UBMS).To obtain hydroxyl group,the films were treated by plasma immersion H~+ ion implantation.The films were then activated by acid solution.After that,aminopropyltriethoxysilane(APTE)was covalently interacted with the hydroxyl on the Ti-O film surface from activation.Then, laminin(Ln)and fibronectin(Fn)were covalently immobilized to the surface by the amidogen of APTE.Scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS),Fourier Transform Infrared spectroscopy (FTIR),Atomic Force Microscopy(AFM)and contact-angle measurement were used to investigate the characteristics of Ti-O films and modified Ti-O film surface.Human umbilical vein endothelial cells(HUVEC)were in vitro seeded to the modified and unmodified Ti-O films surface for evaluating the cell compatibility and endothelialization trend.The results of FTIR indicated that the functional group of hydroxyl was presented onto Ti-O film surface after being pretreated by plasma implantation and acid solution.The peak of O element corresponding to hydroxyl group increased in results of XPS,the contact angle of Ti-O film surface was lower.The results of FTIR and XPS indicated that APTE could be chemically reacted with the hydroxyl of Ti-O film surface.The results of SEM and AFM suggested that the surface roughness was lower after APTE coated and the contact angle of the film surface was increased.The result of FTIR indicated that albumen amideâ… ,and amideâ…¡adsorption peaks were presented after laminin and fibronectin immobilized onto Ti-O films. The peaks were stronger when laminin and fibronectin were immobilized together.The result of immunofluorescence indicated that laminin and fibronectin were immobilized to Ti-O film surface.The contact angle of Ti-O film surface showed no obvious change,and the surface roughness was increased.Cell culture investigation suggested that laminin and fibronectin could enhance HUVEC adhesion and proliferation,and improve the HUVE cell behavior.And the results of HUVECs cultured experiment suggested that Ti-O film which modified by plasma implantation and acid solution and immobilized with Ln and Fn is best.All these results indicated that laminin and fibronectin could be covalently immobilized onto Ti-O film surface by APTE.Laminin and fibronectin can promote the adhesion and growth of endothelial cells.So,it is a good surface endothelialization way to immobilized laminin onto and fibronectin Ti-O film surface.