| Objectives: To investigate the expression of tumorneerosis factor-alpha(TNF-α)and interleukin-6(IL-6)after traumatie brain injury ,and if the secondary brain injury would decrease when the expression was inhibited, in order to provide the theoretie albasis directly for a new approach to the clinical management of tranmatie brain injury.Materials and methods: 56 healthy male Sprague-Dawley rats weighting from 275g to 342g.They were divided randomly into seven groups(A,B,C,D,E,F,G).Every groups had 8 rats.They were anesthetized by 10 percent chloral hydrate from intraperitoneal injection(3ml/kg). When anesthesi a became valid,rats'cupula and occiput wool was snipped. Rats were cut open 4cm scalp follow median line in the cupula and occiput,stripped the soft tissue and periost,exposed bones of skull. Keepping dura undamaged,3mm before lambdoid suture,3mm beside median line of skull, we drilled 5mm round bony window. A free-falling object which caused severe craniocerebral injury was 30 gram,falled from 35centimeter.Then,recording the attacked time,we sew up the scalp and raised them. Rats of G group were disclosed skull only as contrasts without brain contusion. The experimental animals of A,B,C,D,E and F group took samples differently at 24h,72h,120h,l week,2 weeks,3 weeks after operation. All rats were killed by deep anesthesia and lavaged in PB contained 4%formal-dehydum polymerisatum via placing catheter from left ventricle to the major aorta. After satisfied, we stripped the brain tissue,took the zone behide the contusion and opposite sidezone correspondingly as experimental samples. We took the corresponding brain tissue from the contrastive group as contrast. The samples were fixed in 4% formaldehydum polymerisatum for about 24 hours and embedded in the paraffin. The slice thick was about 5um.Immunohistochemical stains(S-P):The processes were performed to examine the expression of TNF-α, and IL-6 in the total samples. The positive reactant of immunohistochemistry was detected in quantitative detection by detect the gray scale.The software package SPSS 11.5 was used for statistical analysis. The immunohistochemical results were reported as mean±standard deviation. The one-way ANOVA and SNK-q test were used to analysis the datas.P<0.05 was considered statistically significant.Results of immunohistochemistry: TNF-αwas stained in endochylema. Every experimentalgroups expressed. The control group expressed by chance. The differences were statistically significant(P<0.01).The expressions of B and C groups were the most obvious. The differences were not statistically significant(P>0.05).The zonesbehide the contusion and opposite side expressed generally inthe C group .The differences were not statistically significant(P>0.05).The expressions in the zones behide the contusionwere much obviously than he opposite side in the A,B,D,E and F groups.The differences were statistically significant(P<0.05).IL-6 was stained in endochylema. Every experimental groups expressed in the zones behide the contusion. The G group didn't expressed obviously. The differences were statistically significant(P<0.01).The expressions of D group were the most obvious. The expressions in the opposite side zone were much obviously in the D,E and F groups. The A,B and C groups expressed slightly in the opposite side zone.The differences were not statistically significant(P>0.05).Conclusions:1.TNF-αexpressed in the earlier period after trauma. The highest expressions days were in the third and the fifth. The differences were not statistically significant(P>0.05).The expressions in the second week and third week were higher obviously than the normal. The overexpressions of TNF-αare existent after severe traumatic head injury. The ex-pressions of the third week were higher than the second week(p<0.05).We consider the TNF-αparticipate reparative process of BI in restoration period after severe craniocerebral injury.2. The highest expressions of IL-6 were at the first week after BI. The expressions of the second week and the t-hird week were also obvious. The time of the highest expressions was later than TNF-α's.TNF-αhave induced the expression of IL-6 possibly. Because of the obvious different expressions in the two sides of the brain tissue within one week,we consider the overexpressions of IL-6 were not existent and it participated reparative pro-cess after BI.3.The highest expressions of TNF-αwere at the third and the fifth day,this was accorded with the crest-time of brain edema clinically. This was close to cerebrospinal fluid detections. The detections of TNF-αin cerebrospinal fluid can be used as an indirect index to reflect intraca inflammation and provid information for clinic.4.Because the tow factors continued to express highly at the third week,they maybe all participate reparative process after BI. |
PDF Full Text Request