The Expression Of TMPRSS4 And OnfFN In Thyroid Carcinoma

Objective:To study the expression of Transmembrane Protease Serine4(TMPRSS4) and Oncofetal Fibronectins(onfFN)mRNA in thyroid carcinoma,adenoma and paraneoplastic tissue by reverse transcriptase- polymerase chain reaction,analysis their correlation with thyroid carcinoma and the different expression in different clinicopathologic parameter,then a further studay was performed to detect the expression of the two marker in FNAB tissue of thyroid nodule by real-time quantitative polymerase chain reaction.Method:80 fresh thyroid samples were collected after operation include 32 malignant and 30 benign,the samples were put into liquid nitrogen as soon as quickly when obtained from operation room,and saved in tissue bank for the next experiment. And 78 FANB samples of thyroid nodule were collected include 45malignant and 33 benign,the aspiration of those samples was guided by ultrasound,some samples put into test tube with TRIzol,others were smeared for cytology check.At the same time collected the case file material of the patients for the resules analysis.First,extract the RNA of fresh samples,and transcribed the RNA into cDNA by RT-PCR,amplificated the cDNA with specific primer of TMPRSS4 and onfFN,and then analysised the expression of the two markers by agarose electrophoresis experiment.Second, dectected expression of the two markers in FANB sample by real-time quantitative polymerase chain reaction.Final,analysis their correlation with thyroid carcinoma and the different expression in different clinicopathologic parameter according the resules of the experiments.Results:The expression rate of TMPRSS4 in carcinoma,adenoma and paraneoplastic tissue was 78.13%,6.67%,5.56 respectively,and onfFN was 82.37%,16.67%,16.67%respectively.There was a statistically significant higher expression of thyroid carcinoma than adenoma and paraneoplastic(p=0.000),and there was a statistically significant positive correlation between expression of TMPRSS4 and onfFN(r=0.413,p=0.019).The expression of TMPRSS4 and onfFN mRNA in TNMâ…¢+â…£and lymph node metastasis was higher than TNMâ… +â…¡and no lymph node metastasis thyroid carcinoma samples(p=0.003,p=0.008 and p=0.015,p=0.05),but,There was no statistically significant in other clinicopathologic parameters.Quantitative results indicated that the microamount of mRNA expression in FNAB samples can be detected by RT-PCR,t-test result demonstrated that TMPRSS4 and onfFN mRNA expression in malignant samples was higher than that in benign samples(t=6.202 and 7.215 respectively,p=0.000),the area under the receiver operating characteristic(AUC)of TMPRSS4 and onfFN was 0.932 and 0.902 (p=0.000),sensitivity and specificity was 92.9%,72.7%and 88.1%,77.3% respectively.Conclusions:The expression of TMPRSS4 and onfFN mRNA in malignant samples was higher than that in benign and paraneoplastic samples,the microamount of mRNA expression in FNAB samples can be detected by real-time quantitative polymerase chain reaction,so we can combined the two markers and FANB to elevate the diagnosis accurate of thyroid carcinoma before operation,which can offered accurate informations for making treatment perscription.The expression of TMPRSS4 and onfFN mRNA in TNMâ…¢+â…£and lymph node metastasis was higher than TNMâ… +â…¡and no lymph node metastasis thyroid carcinoma samples,which demonstrated that the two markers might play a important role in the carcinogenesis, development and metachoresis of thyroid carcinoma,and there was a statistically significant positive correlation between expression of TMPRSS4 and onfFN in thyroid which displayed that their might be a synergistic effect in those processes.