The Expression Of GDNF And It's Receptors In The Salivary Adenoid Cystic Carcinoma

Objective:To study the expression of glial cell line-derived neurotrophic factor(GDNF)and GDNF receptors(GFRα-1,RET)in salivary adenoid cystic carcinoma(SACC)and explore it's relationship with the clini-pathological features and biological behavior of SACC.Materials and Methods:1.10 normal parotid gland,36 salivary adenoid cystic carcinoma (SACC)and 15 Mucoepidermoid carcinoma(MEC)were selected. Clinical information was collected including patient age,location, pathological type,perineural invasion and clinical stage et al.2.Using immuohistochemical SP staining,we examined protein GDNF and it's receptors expression in paraffin slices of 36 salivary adenoid cystic carcinoma.As control,expression of these proteins in 15 MEC and 10 normal parotid gland were also detected.3.Difference of GDNF and it's receptors were analyzed in normal parotid gland,MEC and SACC.Relationships of these proteins expressiom with clini-pathological parameter were analyzed in SACC.Rresult:1,In the gland alveolus cells of normal parotid gland,GDNF and it's receptoe didn't express;In the ductal cells of normal parotid gland,the positive expression rate of GDNF is 50%(5/10),the positive expression rate of GFRα-1 is 40%(4/10).RET didn't find positive expression. 2,In the SACC,the positive expression rates of GDNF is 88.9%(32/36);the positive expression rates of GFRα-1 is 83.3%(30/36); Expression of GDNF and GFRα-1 had no correlation with location, pathological type(P＞0.05),But it had correlation with clinical stage and perineural invasion(P＜0.05),And seven cases of strong positive expression,their GDNF expression had a weakening trend from the nerve to peripheral carcinoma.3,In the Mucoepidermoid carcinoma,GDNF and RET didn't express;the positive expression rate of GFRα-1 is 40.0%(6/15).4.The positive expression rates of GDNF and GFRα-1 has statistical significance between the SACC,Mucoepidermoid carcinoma and normal parotid gland.Conclusions:1,In the normal parotid gland and Mucoepidermoid carcinoma, GDNF and GFRα-1 didn't exoress or had a poor expression,however,In the SACC,GDNF and GFRα-1 expressed significantly;GDNF and it's receptor may be as one of the biological signs with perineural invasion of SACC.2.GDNF and GFRα-1 may play an importent role in the infiltration and perineural invasion of SACC.