5-azacytidine And Trichostatin A Synergistically Induced The Expression Of E-cadherin In A431 Cells

Objectives(1)To observe the differential expression of E-cadherin between human keratinocyte cell line(HaCaT cells)and human epiderma squamous cell carcinoma cell line(A431 cells);(2)To investigate the possible mechanisms of the abnormal E-cadherin expression in A431 cells.Methods(1)HaCaT cells and A431 cells were cultured in vitro;(2)A431 cells were treated with 5-azacytidine(5-azaC)or/and Trichostatin A(TSA);(3)The expression levels of E-cadherin mRNA and protein in cells were investigated by reverse-transcription polymerase chain reaction (RT-PCR)and Western-blot analysis.Results(1)The expression levels of E-cadherin mRNA and protein were significantly decreased in A431 cells compared with that in HaCaT cells (P＜0.05).(2)The expression levels of E-cadherin mRNA and protein were significantly decreased in A431 cells compared with that treated with 5-azaC or TSA respectively(P＜0.05).(3)The expression levels of E-cadherin mRNA and protein were significantly decreased in 5-azaC or TSA treated A431 cells compared with that in cells treatment with these two drugs(P＜0.05).ConclusionsHypermethylation of gene and deacetylation of histone protein maybe have synergistic effect on the decreased expression of E-cadherin in A431 cells.