Study Of The Correlationship Between C-reactive Protein Gene Polymorphism And Cerebral Infarction

Objective To investigate the gene frequencies of CRP -717A＞G variants and to study the relationship between CRP single nucleotide polymorphism(SNP)and the concentrations of serum hs-CRP(High Sensitivity CRP,hs-CRP)and cerebral infarction in Chinese Han population.Method A case-control study including 84 patients with cerebral infarction,as well as 76 nomal control subjectswas carried out in this research.-717A＞G gene polymorphisms were detected by direct sequencing within the promoter region of CRP gene,and the levels of serum high sensivity C-reactive protein were measured with latex particle enhanced immunoturbidimetry.Experimental results were analyzed with Spss 15.0.Results1.The -717A＞G genotype distribution in Han nationality of Hunan Province China was AA 66.3%,AG 32.5%and GG 1.3%,respectively. Allele frequencies for A and G were 82.55%,17.45%respectively.2.In cerebral infarction group,the AA gene frequency of CRP -717A＞G gene cite is much higher than that of control group,and the difference is significant(P＜0.05).3.The serum level of hs-CRP of the AA gene phenotype of CRP -717A＞G gene cite in the control and cerebral infarction group are both higher than that of AG/GG gene phenotype,but the difference is not significant.4.Binary logistic regression analysis suggests that -717 AG+GG gene phenotype has a protective effect on preventing the development of cerebral infarction.Conclusion1.This study observated common genetic variants distribution of CRP -717A＞G in Chinese Han population in HuNan.2.The level of serum hs-CRP reflected the severity degree of cerebral infarction.3.CRP -717A＞G genotype in the two groups were not correlated with the serum hs-CRP.4.The-717 GG+GA genotype had an independent protective effect on strokes.